Deuterium nuclear magnetic resonance measurements of glycoproteins which have been specifically deuterated either at selected carbohydrate or lysine residues: determination of motional correlation times
- Univ. of British Columbia, Vancouver
The results of this work should demonstrate that the combination of highly selective deuteration and /sup 2/HNMR studies can lead to potentially valuable motional information. Deuterium at C-7 of the sialic acid residues of glycoproteins was achieved by using limited quantities of aqueous sodium metaperiodate to cleave selectively the C-7, C-8, C-9 triol moiety followed by reduction using sodium borodeuteride. The /sup 2/HNMR spectrum shows the sensitivity which can be obtained. The substrate is bovine submaxillary mucin, which has potentially one deuterium substituent attached to each of the 300 sialic acid residues per molecule. Selected monodeuteration of C-6 at the terminal galactose residues of suitable glycoproteins was effected using sodium borodeuteride as the reductant. The lysine groups of fetuin were reductively aminated with hexadeuteroacetone on lysine residues. An approximate estimation of the upper limit for the overall motional times can be obtained from the 1/2 height time width of the deuterium resonances. 3 figures.
- OSTI ID:
- 6062892
- Journal Information:
- J. Am. Chem. Soc.; (United States), Vol. 101:10
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
59 BASIC BIOLOGICAL SCIENCES
ACETONE
CHEMICAL REACTIONS
BOROHYDRIDES
DEUTERIUM
ISOTOPE EFFECTS
ISOTOPIC EXCHANGE
GALACTOSE
LABELLING
LYSINE
PERIODATES
SIALIC ACID
CATTLE
COMPARATIVE EVALUATIONS
DEUTERIUM COMPOUNDS
EXPERIMENTAL DATA
GLUCOPROTEINS
ISOLATED VALUES
NUCLEAR MAGNETIC RESONANCE
RESIDUES
SENSITIVITY
SODIUM COMPOUNDS
ALDEHYDES
ALKALI METAL COMPOUNDS
AMINO ACIDS
ANIMALS
BORON COMPOUNDS
CARBOHYDRATES
CARBOXYLIC ACIDS
DATA
DATA FORMS
DOMESTIC ANIMALS
HALOGEN COMPOUNDS
HEXOSES
HYDROGEN COMPOUNDS
HYDROGEN ISOTOPES
INFORMATION
IODINE COMPOUNDS
ISOTOPES
KETONES
LIGHT NUCLEI
MAGNETIC RESONANCE
MAMMALS
MONOSACCHARIDES
NUCLEI
NUMERICAL DATA
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
POLYSACCHARIDES
PROTEINS
RESONANCE
RUMINANTS
SACCHARIDES
STABLE ISOTOPES
VERTEBRATES
400302* - Organic Chemistry- Isotope Effects- (-1987)
400303 - Organic Chemistry- Isotope Exchange & Isotope Separation- (-1987)
400301 - Organic Chemistry- Chemical & Physicochemical Properties- (-1987)
550200 - Biochemistry