In vitro labeling of the sialic acid moiety of glycoconjugates with carbon-14

Journal Article · Thu Mar 01 00:00:00 EST 1984 · Anal. Biochem.; (United States)

DOI:https://doi.org/10.1016/0003-2697(84)90109-X· OSTI ID:5696334

Carubelli, R; Wen, G; McCaffree, D R

Labeling of sialoglycoproteins with carbon-14 in vitro was performed by reacting the aldehyde groups, generated by mild periodate oxidation of the terminal sialyl groups, with /sup 14/C-labeled sodium cyanide to produce the labeled cyanohydrin derivatives (Kiliani reaction). Labeling with tritium was carried out by reduction by the aldehyde groups generated on the sialyl residues with /sup 3/H-labeled sodium borohydride following standard procedures. The behavior of both types of labeled specimens of fetuin and ovine submaxillary mucin, individually and in mixtures, was investigated by gel-filtration chromatography, gel electrophoresis, and cesium bromide gradient ultracentrifugation. The labeled sialyl residues were subjected to partial characterization; color yield with the resorcinol and thiobarbituric acid reagents, behavior on ion-exchange chromatography, and susceptibility to mild acid and enzymatic hydrolyses. In addition to these model glycoproteins, this procedure was also utilized to label the sialoglycoproteins present in human tracheobronchial secretions collected from normal subjects and patients with chronic bronchitis. The potential uses of this approach for comparative studies of normal and pathological sialoglycoconjugates available in minute amounts is described. The extension of this approach to the labeling of the galactosyl and N-acetylgalactosaminyl moieties of glycoconjugates following treatment with galactose oxidase is outlined. 21 references.

• Research Organization: Univ. of Oklahoma, Oklahoma City
• OSTI ID: 5696334
• Journal Information: Anal. Biochem.; (United States), Vol. 137:2
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
59 BASIC BIOLOGICAL SCIENCES
GLUCOPROTEINS
LABELLING
CARBON 14 COMPOUNDS
COMPARATIVE EVALUATIONS
ELECTROPHORESIS
GEL PERMEATION CHROMATOGRAPHY
MUCOPROTEINS
PATIENTS
RESPIRATORY SYSTEM DISEASES
SIALIC ACID
TRITIUM COMPOUNDS
ULTRACENTRIFUGATION
CARBOHYDRATES
CENTRIFUGATION
CHROMATOGRAPHY
DISEASES
LABELLED COMPOUNDS
ORGANIC COMPOUNDS
POLYSACCHARIDES
PROTEINS
SACCHARIDES
SEPARATION PROCESSES
550601* - Medicine- Unsealed Radionuclides in Diagnostics
550201 - Biochemistry- Tracer Techniques