Plant tissue hybrid electrode for determination of phosphate and fluoride
A biosensor for inorganic phosphate and fluoride has been developed by coupling a potato (Solanum tuberosum) tissue slice and immobilized glucose oxidase with a Clark oxygen electrode. Measurement is based on the inhibition by either ion of potato acid phosphates catalyzed glucose 6-phosphate hydrolysis. The precision is 1.7% and 6.5% and the lower detection limit 2.5 X 10/sup -5/ M and 1 X 10/sup -4/ M for phosphate and fluoride, respectively. For phosphate determination the hybrid sensor is stable for 28 days or 300 assays. With a higher limit of detection the sensor can be applied in a commercial enzyme electrode based device. Its application for phosphate determination in fertilizer and urine samples is described.
- Research Organization:
- Central Inst. of Molecular Biology, Berlin-Buch, East Germany
- OSTI ID:
- 6062795
- Journal Information:
- Anal. Chem.; (United States), Vol. 56:9
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
FERTILIZERS
QUANTITATIVE CHEMICAL ANALYSIS
FLUORIDES
AMPEROMETRY
POTENTIOMETRY
PHOSPHATES
URINE
ELECTRODES
ENZYMES
EXPERIMENTAL DATA
POTATOES
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BODY FLUIDS
CHEMICAL ANALYSIS
DATA
FLUORINE COMPOUNDS
FOOD
HALIDES
HALOGEN COMPOUNDS
INFORMATION
MATERIALS
NUMERICAL DATA
OXYGEN COMPOUNDS
PHOSPHORUS COMPOUNDS
PLANTS
TITRATION
TUBERS
VEGETABLES
WASTES
400102* - Chemical & Spectral Procedures