Enzyme monolayer- and bilayer-modified tin oxide electrodes for the determination of hydrogen peroxide and glucose
- Univ. of Tokyo (Japan)
An enzyme-based amperometric biosensor for hydrogen peroxide was developed, employing a horseradish peroxidase (HRP) monolayer covalently attached to a tin oxide electrode and a dissolved electron mediator. The sensor can determine hydrogen peroxide at levels down to 10{sup {minus}8} M and can be applied to a flow system. Stability of the electrode, kinetics of the surface process, and the efficiencies of different mediators were studied. As an extension, glucose oxidase (GOx) was chemically bound to the HRP-modified electrode. A GOx/HRP bilayer-modified electrode thus obtained exhibits much better performance in glucose detection limit, sensitivity, and response speed than previously reported GOx monolayer-modified electrodes.
- OSTI ID:
- 5938497
- Journal Information:
- Analytical Chemistry (Washington); (USA), Vol. 61:21; ISSN 0003-2700
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
GLUCOSE
QUANTITATIVE CHEMICAL ANALYSIS
HYDROGEN PEROXIDE
ELECTRODES
ENZYMES
MEASURING INSTRUMENTS
MEASURING METHODS
TIN OXIDES
ALDEHYDES
CARBOHYDRATES
CHALCOGENIDES
CHEMICAL ANALYSIS
HEXOSES
HYDROGEN COMPOUNDS
MONOSACCHARIDES
ORGANIC COMPOUNDS
OXIDES
OXYGEN COMPOUNDS
PEROXIDES
SACCHARIDES
TIN COMPOUNDS
400102* - Chemical & Spectral Procedures