Determination of metals at the microgram-per-liter level in blood serum by simultaneous multielement atomic absorption spectrometry with graphite furnace atomization

Journal Article · Tue Jan 01 04:00:00 EST 1985 · Anal. Chem.; (United States)

DOI:https://doi.org/10.1021/ac00279a005· OSTI ID:6062429

Lewis, S A; O'Haver, T C; Harnly, J M

A method is described for the simultaneous determination of seven metals - Al, Co, Cr, Mn, Mo, Ni, and V - in blood serum using a simultaneous, multielement atomic absorption spectrometer (SIMAAC) and graphite furnace atomization. Our original intention was to determine two additional metals - Pb and Sn - however, the high ashing temperature needed resulted in premature loss of these volatile metals. Serum samples (2 mL) are dry-ashed with 20 ..mu..L of Mg(NO/sub 3/)/sub 2/.6H/sub 2/O (20 g/L) as an ashing aid/matrix modifier. The organic-free residue is reconsituted with 0.5 mL of nitric acid (50 mL/L), allowing for a fourfold concentration step. The addition of Mg(NO/sub 3/)/sub 2/ permits a graphite furnace ashing temperature of 1300/sup 0/C. Atomization from a L'vov platform with a fast heating rate and the use of peak area measurements permit calibration with acidifed aqueous standards. A well-characterized bovine serum pool is used to establish the accuracy and precision of the method. Serum samples from 30 fasting adult volunteers, drawn under controlled conditions, are analyzed to assess the efficacy of this method to determine these seven metals over the ranges normally encountered in human sera. 16 references, 1 figure, 8 tables.

• Research Organization: Univ. of Maryland, College Park
• OSTI ID: 6062429
• Journal Information: Anal. Chem.; (United States), Journal Name: Anal. Chem.; (United States) Vol. 57:1; ISSN ANCHA
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400104* -- Spectral Procedures-- (-1987)
551000 -- Physiological Systems
59 BASIC BIOLOGICAL SCIENCES
ABSORPTION SPECTROSCOPY
ALUMINIUM
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CHEMISTRY
BLOOD SERUM
BODY FLUIDS
CATTLE
CHEMICAL ANALYSIS
CHROMIUM
COBALT
DATA
DOMESTIC ANIMALS
ELEMENTS
EXPERIMENTAL DATA
INFORMATION
MAMMALS
MANGANESE
MATERIALS
METALS
MOLYBDENUM
NICKEL
NUMERICAL DATA
QUANTITATIVE CHEMICAL ANALYSIS
RUMINANTS
SPECTROSCOPY
TRANSITION ELEMENTS
VANADIUM
VERTEBRATES