Simultaneous multielement analysis of microliter quantities of serum for copper, iron, and zinc by graphite furnace atomic absorption spectrometry

Journal Article · Wed Aug 01 04:00:00 EDT 1984 · Anal. Chem.; (United States)

DOI:https://doi.org/10.1021/ac00273a026· OSTI ID:6057708

Lewis, S A; O'Haver, T C; Harnly, J M

A method is described for the simultaneous determination of Cu, Fe, and Zn in 25 ..mu..L of serum, using simultaneous, multielement atomic absorption spectrometry (SIMAAC) and graphite furnace atomization. Peak height measurements are more adversely affected than peak area measurements by both the organic and inorganic constituents in serum. Peak area measurements are significantly more precise and accurate than peak height measurements. Determinations are carried out at a 1:21 dilution of serum, in the concentration range with the optimum precision and minimum carryover contamination and memory effects for these elements, using SIMAAC. The serum specimens are diluted with Triton X-100 (10 mL/L), 10 mM nitric acid, and 30 mM ammonium nitrate. The use of peak area measurements and atomization from a L'vov platform permits calibration with acidified aqueous standards. A commercially available, protein-based reference material, Cation-Cal (American Dade) and a bovine serum pool are used as controls. The method is shown to have good correlation to comparative methods (single-element flame AAS after deproteinization) for the analysis of 37 serum samples.

• Research Organization: Univ. of Maryland, College Park
• OSTI ID: 6057708
• Journal Information: Anal. Chem.; (United States), Journal Name: Anal. Chem.; (United States) Vol. 56:9; ISSN ANCHA
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400104* -- Spectral Procedures-- (-1987)
ABSORPTION SPECTROSCOPY
CHEMICAL ANALYSIS
COMPARATIVE EVALUATIONS
COPPER
DATA
DISPERSIONS
ELEMENTS
EXPERIMENTAL DATA
INFORMATION
IRON
METALS
MIXTURES
NUMERICAL DATA
QUANTITATIVE CHEMICAL ANALYSIS
SOLUTIONS
SPECTROSCOPY
TRANSITION ELEMENTS
ZINC