Effects of lead nitrate on the central nervous system of the chick embryo. II. Electron microscopy and histochemistry: peroxisomes

Journal Article · · Growth; (United States)

OSTI ID:6061538

De Gennaro, L D

The deleterious effects of lead on the developing central nervous system were observed in chick embryos that were exposed to lead nitrate at 10 days of incubation. At 16 days, cervical, thoracic, and lumbar regions of the spinal cord were fixed in gluteraldehyde and processed for electron microscopy, either directly or following incubation in media containing 3,3-diaminobenzidine and hydrogen peroxide for the histochemical detection of catalase-reactive peroxisomes. The results indicate that peroxisomes are not destroyed as are other components of the neuropil after lead exposure. Catalase-reactive peroxisomes appear to increase in number in the spinal cords of lead-treated embryos suggesting that these organelles may play a part in the response of glial cells to metabolic alterations induced by lead. Further experimentation using the chick embryo as an animal model for studies of elad-induced neuropathy is encouraged.

• Research Organization: Le Moyne College, Syracuse, NY
• OSTI ID: 6061538
• Journal Information: Growth; (United States), Journal Name: Growth; (United States) Vol. 51:2; ISSN GROWA
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BIOLOGICAL MODELS
BIRDS
CELL CONSTITUENTS
CENTRAL NERVOUS SYSTEM
CHICKENS
DISEASES
ELECTRON MICROSCOPY
ELEMENTS
EMBRYOS
FOWL
HISTOLOGY
LEAD
METALS
MICROSCOPY
NERVOUS SYSTEM
NERVOUS SYSTEM DISEASES
PATHOGENESIS
PATHOLOGICAL CHANGES
TOXICITY
VERTEBRATES