Hypoxia/reoxygenation increases the permeability of endothelial cell monolayers: Role of oxygen radicals

Journal Article · Sun Dec 31 23:00:00 EST 1989 · Free Radical Biology and Medicine; (USA)

DOI:https://doi.org/10.1016/0891-5849(90)90031-D· OSTI ID:6061050

Inauen, W; Payne, D K; Kvietys, P R; Granger, D N ^[1]

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1. Louisiana State Univ. Medical Center, Shreveport (USA)

We assessed the effect of hypoxia/reoxygenation on 14C-albumin flux across endothelial monolayers. Cultured bovine pulmonary artery endothelial cells were grown to confluence on nitrocellulose filters (pore size 12 microns). The endothelialized filters were mounted in Ussing-type chambers which were filled with cell culture medium (M 199). Equimolar amounts (33 nM) of 14C-labeled and unlabeled albumin were added to the hot and cold chambers, respectively. The monolayers were then exposed to successive periods (90 min) of normoxia (pO2 145 mmHg), hypoxia (pO2 20 mmHg), and reoxygenation (pO2 145 mmHg). A gas bubbling system was used to control media pO2 and to ensure adequate mixing. Four aliquots of culture media were taken during each period in order to calculate the 14C-albumin permeability across the endothelialized filter. In some experiments, either the xanthine oxidase inhibitor, oxypurinol (10 microM), or superoxide dismutase (600 U/mL), was added to the media immediately prior to the experiments. As compared to the normoxic control period, albumin permeability was 1.5 times higher during hypoxia (p less than 0.01) and 2.3 times higher during reoxygenation (p less than 0.01). The reoxygenation-induced increase in albumin permeability was prevented by either oxypurinol or superoxide dismutase. These data indicate that xanthine oxidase-derived oxygen radicals contribute to the hypoxia/reoxygenation-induced endothelial cell dysfunction. The altered endothelial barrier function induced by hypoxia/reoxygenation is consistent with the microvascular dysfunction observed following reperfusion of ischemic tissues.

• OSTI ID: 6061050
• Journal Information: Free Radical Biology and Medicine; (USA), Journal Name: Free Radical Biology and Medicine; (USA) Vol. 9:3; ISSN FRBME; ISSN 0891-5849
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALBUMINS
ANIMAL TISSUES
ANOXIA
AROMATICS
AZAARENES
BIOLOGICAL EFFECTS
BIOLOGICAL FUNCTIONS
BODY
CARBON 14 COMPOUNDS
CELL CONSTITUENTS
CELL MEMBRANES
ELEMENTS
ENDOTHELIUM
ENZYME INHIBITORS
ENZYMES
FUNCTIONS
HETEROCYCLIC COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MEMBRANE TRANSPORT
MEMBRANES
NONMETALS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
OXIDOREDUCTASES
OXYGEN
PERMEABILITY
PROTEINS
PURINES
RADICALS
SUPEROXIDE DISMUTASE
TISSUES
TRACER TECHNIQUES
XANTHINES