Characterization of the H4IIE rate hepatoma cell bioassay as a tool for assessing toxic potency of planar halogenated hydrocarbons in environmental samples

Journal Article · Tue Jan 01 04:00:00 EST 1991 · Environmental Science and Technology; (USA)

DOI:https://doi.org/10.1021/es00013a007· OSTI ID:6059635

Tillitt, D E; Giesy, J P ^[1]; Ankley, G T ^[2]

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1. Michigan State Univ., East Lansing (USA)
2. Environmental Protection Agency, Duluth, MN (USA)

An in vitro system, the H4IIE rat hepatoma cell bioassay, was characterized for use in assessing the overall toxic potency of PCBs, PCDDs, and PCDFs in extracts from environmental samples. This in vitro bioassay of cytochrome P450IA1 catalytic activity in the H4IIE cells in response to planar halogenated hydrocarbons (PHHs) was repeatable over time and standards were reproducible among laboratories when dosing conditions were similar. Three common extraction/cleanup procedures tested had no adverse affect on the response of the cells and biogenic interferences were not encountered. Comparison of the response of the H4IIE cells to extracts was calibrated against their response to the standard, 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). This method of calibration proved to be effective for quantitation of known amounts of PHHs spiked into a sample matrix. The potential utility of this bioassay is as an integrative tool to assess the toxic potency of complex mixtures of PHHs. The results of this bioassay can complement chemical residue analysis and direct the need for such analysis, as well as aid in the interpretation of biological effects data from environmental studies.

• OSTI ID: 6059635
• Journal Information: Environmental Science and Technology; (USA), Journal Name: Environmental Science and Technology; (USA) Vol. 25:1; ISSN ESTHA; ISSN 0013-936X
• Country of Publication: United States
• Language: English

Related Subjects

54 ENVIRONMENTAL SCIENCES
540320* -- Environment
Aquatic-- Chemicals Monitoring & Transport-- (1990-)
560300 -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
AROMATICS
BIOASSAY
CHLORINATED AROMATIC HYDROCARBONS
DATA
DIOXIN
EXPERIMENTAL DATA
HALOGENATED AROMATIC HYDROCARBONS
HETEROCYCLIC COMPOUNDS
IN VITRO
INFORMATION
MAMMALS
MEASURING METHODS
NUMERICAL DATA
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
RATS
RODENTS
TOXICITY
VERTEBRATES