Characterization studies of H4IIE bioassay for assessment of planar halogenated hydrocarbons in fish and wildlife

Thesis/Dissertation · Sun Jan 01 04:00:00 EST 1989

OSTI ID:5543137

Tillitt, D E

Planar halogenated hydrocarbons (PHHs) are a group of environmental contaminants which include polychlorinated biphenyls (PCBs), polychlorinated dibenzo-p-dioxins (PCDDs), and polychlorinated dibenzofurans (PCDFs). PHH contamination in the environment is of particular concern because these compounds are extremely toxic, bioaccumalate in aquatic food chains, and are relatively persistent. Even though there are hundreds of PHHs, they are approximate isostereomers, produce the same characteristic toxic symptoms in a variety of phyla, and are believed to act through the same receptor-based mode of action. These similarities of PHH have lead to the development of quantitative structure-activity relationships (SAR) for PHHs in both in vivo and in vitro systems. One particularly interesting and potentially useful SAR has been observed between the induction potency of individual PHH congeners in H4IIE rat hepatoma cells toward ethoxyresorufin-O-deethylase activity and their in vivo toxicity in rats. This relationship between in vitro induction potency and in vivo toxic potency to the whole organism, along with the ability of the H4IIE cells to act as integrators at the cellular level, has led to their use in assessment of complex mixtures of PHH in the environment. The studies reported here focused on characterization of the H4IIE bioassay for use as a bioanalytical monitoring tool for the assessment of toxicity of PHH mixture in fish and wildlife. Results of these studies indicate that the H4IIE bioassay is precise, has good reproducibility, is reasonably accurate when tested with fortified samples, and has no discernable interferences caused by common extraction protocols or sample matrices. The H4IIE bioassay was used to screen PHH mixture in eggs of colonial fish-eating waterbirds from around the Great Lakes.

• Research Organization: Michigan State Univ., East Lansing, MI (United States)
• OSTI ID: 5543137
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
AQUATIC ORGANISMS
AROMATICS
BENZOFURANS
BIOASSAY
BIOLOGICAL ACCUMULATION
CHLORINATED AROMATIC HYDROCARBONS
CONTAMINATION
DIOXIN
DISEASES
ENZYMES
FISHES
FOOD CHAINS
FURANS
HALOGENATED AROMATIC HYDROCARBONS
HEPATOMAS
HETEROCYCLIC COMPOUNDS
MAMMALS
NEOPLASMS
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
OXIDOREDUCTASES
RATS
RODENTS
STRUCTURE-ACTIVITY RELATIONSHIPS
TOXICITY
VERTEBRATES