Effects of in vitro UVA irradiation and PUVA treatment on membrane fatty acids and activities of antioxidant enzymes in human keratinocytes

Journal Article · Fri Feb 01 04:00:00 EST 1991 · Journal of Investigative Dermatology; (USA)

DOI:https://doi.org/10.1111/1523-1747.ep12462271· OSTI ID:6058456

Jansen, C T; Puntala, A; Ahotupa, M ^[1]

---

1. Univ. of Turku (Finland)

Human Keratinocytes (NCTC 2544) in culture were exposed to either plain ultraviolet A (UVA) irradiation or to 8-methoxypsoralen plus UVA (PUVA) treatment. Lipid peroxidation, activities of antioxidant enzymes, and percentage amounts of 14C-arachidonic acid in various cellular lipid subclasses and in the culture medium were measured. Both UVA irradiation and PUVA treatment induced significant changes in the distribution of arachidonic acid and increased the liberation of arachidonic acid from membrane phospholipids. At 24 h after either UVA irradiation or PUVA treatment the formation of thiobarbituric acid reactive material was significantly increased, whereas the amount of conjugated dienes was unaffected. The activities of the antioxidant enzymes, catalase and superoxide dismutase, were already significantly decreased at 0.5 h after UVA irradiation or PUVA treatment. The enzyme activities were partially restored during the following 24 h incubation. From the present study, we suggest that in keratinocytes both plain UVA irradiation and PUVA treatment induce changes in the distribution of membrane fatty acids and cause an impairment in the enzymic defense system against oxidative stress.

• OSTI ID: 6058456
• Journal Information: Journal of Investigative Dermatology; (USA), Journal Name: Journal of Investigative Dermatology; (USA) Vol. 96:2; ISSN 0022-202X; ISSN JIDEA
• Country of Publication: United States
• Language: English

Related Subjects

550201 -- Biochemistry-- Tracer Techniques
560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
ARACHIDONIC ACID
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CATALASE
CELL CONSTITUENTS
CELL MEMBRANES
DISTRIBUTION
ELECTROMAGNETIC RADIATION
ENZYME ACTIVITY
ENZYMES
EPIDERMIS
EPITHELIUM
ESTERS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIPIDS
MAMMALS
MAN
MEMBRANES
METABOLISM
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
OXIDOREDUCTASES
PEROXIDASES
PHOSPHOLIPIDS
PRIMATES
RADIATION EFFECTS
RADIATIONS
SKIN
SUBCELLULAR DISTRIBUTION
SUPEROXIDE DISMUTASE
TISSUES
TRACER TECHNIQUES
ULTRAVIOLET RADIATION
VERTEBRATES