Diabetes-induced increases in sup 131 I-albumin permeation are unaffected by essential fatty acid depletion

Conference · Mon Feb 26 00:00:00 EST 1990 · FASEB Journal (Federation of American Societies for Experimental Biology); (United States)

OSTI ID:6057396

Williamson, J R; Lefkowith, J B; Chang, K; Tilton, R G ^[1]

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1. Washington Univ., St. Louis, MO (United States)

The authors assessed effects of essential fatty acid deficiency (EFAD) on regional {sup 131}I-albumin permeation in diabetic and age-matched control rats. Male, Sprague-Dawley rats (50-75 g) were randomized into EFAD diet or normal diet groups. Three months later, diabetes was induced in one half of the rats in each group by injecting i.v. 35-45 mg/kg b.w. streptozotocin. One month later, {sup 131}I-albumin clearance ({mu} g plasma/g tissue/minute) was assessed as described previously (Circ Res 64;890, 1989). Within controls, EFAD decreased body weight gain 28% but did not affect control values for plasma glucose (118{plus minus}8 (SD) mg/dl) or glycosylated hemoglobin (1.33{plus minus}0.22 % of total hemoglobin). In normal diet and EFAD diabetics, plasma glucose (535{plus minus}64 and 419{plus minus}161, respectively) and glycosylated hemoglobin (4.38{plus minus}0.97 and 2.97{plus minus}1.69) were increased significantly versus controls. Diabetes increased {sup 131}I-albumin clearance in retinal (5.1x controls), choroid (3.4x), anterior uvea (2.7x), aorta (3.5x), and sciatic nerve (2.2x). No differences were evident in tissue {sup 131}I-albumin clearances between both control groups or both diabetic groups. These results suggest that essential fatty acids do not modulate diabetes-induced changes in endothelial cell barrier function.

• OSTI ID: 6057396
• Report Number(s): CONF-9104107-; CODEN: FAJOE
• Journal Information: FASEB Journal (Federation of American Societies for Experimental Biology); (United States), Vol. 4:3; Conference: 75. annual meeting of the Federation of American Societies for Experimental Biology (FASEB), Atlanta, GA (United States), 21-25 Apr 1991; ISSN 0892-6638
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ALBUMINS
BIOCHEMICAL REACTION KINETICS
NUTRITIONAL DEFICIENCY
BIOLOGICAL EFFECTS
ANIMAL CELLS
CARBOXYLIC ACIDS
CLEARANCE
DIABETES MELLITUS
DIET
ENDOTHELIUM
GLYCEROL
HEMOGLOBIN
IODINE 131
RATS
TRACER TECHNIQUES
ALCOHOLS
ANIMAL TISSUES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
DAYS LIVING RADIOISOTOPES
DISEASES
ENDOCRINE DISEASES
GLOBINS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
MAMMALS
METABOLIC DISEASES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PIGMENTS
PORPHYRINS
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RODENTS
TISSUES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques