GMP-140 binds to a glycoprotein receptor on human neutrophils: Evidence for a lectin-like interaction

Journal Article · Fri Feb 01 04:00:00 EST 1991 · Journal of Cell Biology

DOI:https://doi.org/10.1083/jcb.112.3.491· OSTI ID:6056737

Moore, K L; Varki, A; McEver, R P ^[1]

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1. Univ. of Oklahoma Health Sciences Center, Oklahoma City (USA)

GMP-140 is a rapidly inducible receptor for neutrophils and monocytes expressed on activated platelets and endothelial cells. It is a member of the selectin family of lectin-like cell surface molecules that mediate leukocyte adhesion. We used a radioligand binding assay to characterize the interaction of purified GMP-140 with human neutrophils. Unstimulated neutrophils rapidly bound (¹²⁵I)GMP-140 at 4 degrees C, reaching equilibrium in 10-15 min. Binding was Ca²⁺ dependent, reversible, and saturable at 3-6 nM free GMP-140 with half-maximal binding at approximately 1.5 nM. Receptor density and apparent affinity were not altered when neutrophils were stimulated with 4 beta-phorbol 12-myristate 13-acetate. Treatment of neutrophils with proteases abolished specific binding of (¹²⁵I)GMP-140. Binding was also diminished when neutrophils were treated with neuraminidase from Vibrio cholerae, which cleaves alpha 2-3-, alpha 2-6-, and alpha 2-8-linked sialic acids, or from Newcastle disease virus, which cleaves only alpha 2-3- and alpha 2-8-linked sialic acids. Binding was not inhibited by an mAb to the abundant myeloid oligosaccharide, Le^x (CD15), or by the neoglycoproteins Le^x-BSA and sialyl-Le^x-BSA. We conclude that neutrophils constitutively express a glycoprotein receptor for GMP-140, which contains sialic acid residues that are essential for function. These findings support the concept that GMP-140 interacts with leukocytes by a lectin-like mechanism.

• OSTI ID: 6056737
• Journal Information: Journal of Cell Biology, Journal Name: Journal of Cell Biology Journal Issue: 3 Vol. 112; ISSN 0021-9525
• Publisher: Rockefeller University Press
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
560300 -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADHESION
ANIMALS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD PLATELETS
BODY FLUIDS
CARCINOGENS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ENZYMES
ESTERS
GLYCOPROTEINS
GLYCOSYL HYDROLASES
HYDROLASES
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LECTINS
LEUKOCYTES
LIGANDS
MAMMALS
MAN
MATERIALS
MEMBRANE PROTEINS
MONOCLONAL ANTIBODIES
NEUTROPHILS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PEPTIDE HYDROLASES
PHORBOL ESTERS
PRIMATES
PROTEINS
RADIOASSAY
RADIOISOTOPES
REACTION KINETICS
RECEPTORS
TRACER TECHNIQUES
VERTEBRATES