The opposing effects of calmodulin, adenosine 5 prime -triphosphate, and pertussis toxin on phorbol ester induced inhibition of atrial natriuretic factor stimulated guanylate cyclase in SK-NEP-1 cells

Journal Article · Tue Jan 01 00:00:00 EST 1991 · Life Sciences; (USA)

DOI:https://doi.org/10.1016/0024-3205(91)90508-9· OSTI ID:6055496

Sekiya, M; Frohlich, E D; Cole, F E ^[1]

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1. Alton Ochsner Medical Foundation, New Orleans, LA (USA)

In the present study, we investigated the effects of calmodulin, adenosine 5{prime}-triphosphate (ATP) and pertussis toxin (PT) on phorbol ester (PMA) induced inhibition of ANF-stimulated cyclic GMP formation in cells from the human renal cell line, SK-NEP-1. PMA inhibited ANF-stimulated guanylate cyclase activity in particulate membranes by about 65%. Calmodulin reversed this inhibition in a dose dependent manner. ATP potentiated Mg++ but not Mn++ supported guanylate cyclase activity. In PMA treated membranes, ATP potentiating effects were abolished. PMA also inhibited ANF-stimulated cGMP accumulation, but pretreatment with PT prevented this PMA inhibition. PT did not affect basal or ANF-stimulated cGMP accumulation. In conclusion, these results demonstrated that PMA inhibited ANF stimulation of particulate guanylate cyclase in opposition to the activating effects of calmodulin or ATP in SK-NEP-1 cells. The protein kinase C inhibitory effects appeared to be mediated via a PT-sensitive G protein.

• OSTI ID: 6055496
• Journal Information: Life Sciences; (USA), Vol. 48:11; ISSN 0024-3205
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ATP
BIOLOGICAL EFFECTS
CALMODULIN
CYCLASES
ENZYME ACTIVITY
PHORBOL ESTERS
TOXINS
ANIMAL CELLS
BIOSYNTHESIS
CELL MEMBRANES
INHIBITION
KIDNEYS
MAGNESIUM COMPOUNDS
MAN
MANGANESE COMPOUNDS
NUCLEOTIDES
ALKALINE EARTH METAL COMPOUNDS
ANIMALS
ANTIGENS
BODY
CARCINOGENS
CELL CONSTITUENTS
ENZYMES
ESTERS
LYASES
MAMMALS
MATERIALS
MEMBRANES
ORGANIC COMPOUNDS
ORGANS
PRIMATES
PROTEINS
SYNTHESIS
TOXIC MATERIALS
TRANSITION ELEMENT COMPOUNDS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology