Mechanism of activation of particulate guanylate cyclase by atrial natriuretic peptide as deduced from radiation inactivation analysis

Journal Article · Mon Jan 30 23:00:00 EST 1989 · Biochem. Biophys. Res. Commun.; (United States)

DOI:https://doi.org/10.1016/S0006-291X(89)80092-0· OSTI ID:6462345

Ohuchi, S; Hagiwara, H; Ishido, M; Fujita, T; Kume, T; Ishigaki, I; Hirose, S

The interaction between the receptor (Rc) for atrial natriuretic peptide (ANP) and the effector enzyme particulate guanylate cyclase (GC) has been studied by radiation inactivation. Irradiation of bovine lung membranes produced an increase in GC activity at low radiation doses followed by a dose-dependent reduction at higher doses. This deviation from linearity in the inactivation curve disappeared when lung membranes were pretreated with ANP. Essentially identical results were also obtained with adrenal membranes. Based on these radiation inactivation data, the following dissociative mechanism of activation of particulate guanylate cyclase by ANP has been proposed: Rc.GC(inactive) + ANP----Rc.ANP + GC(active).

• Research Organization: Tokyo Institute of Technology (Japan)
• OSTI ID: 6462345
• Journal Information: Biochem. Biophys. Res. Commun.; (United States), Journal Name: Biochem. Biophys. Res. Commun.; (United States) Vol. 158:2; ISSN BBRCA
• Country of Publication: United States
• Language: English

Related Subjects

560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADRENAL GLANDS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BODY
CATTLE
CELL CONSTITUENTS
CELL MEMBRANES
CYCLASES
DOMESTIC ANIMALS
DOSE-RESPONSE RELATIONSHIPS
ENDOCRINE GLANDS
ENZYME ACTIVITY
ENZYMES
GLANDS
IN VITRO
LUNGS
LYASES
MAMMALS
MEMBRANE PROTEINS
MEMBRANES
ORGANIC COMPOUNDS
ORGANS
PEPTIDES
PROTEINS
RADIATION EFFECTS
RADIOSENSITIVITY EFFECTS
RECEPTORS
RESPIRATORY SYSTEM
RUMINANTS
VERTEBRATES