Mechanism of activation of particulate guanylate cyclase by atrial natriuretic peptide as deduced from radiation inactivation analysis

Journal Article · Tue Jan 31 00:00:00 EST 1989 · Biochem. Biophys. Res. Commun.; (United States)

DOI:https://doi.org/10.1016/S0006-291X(89)80092-0· OSTI ID:6462345

Ohuchi, S; Hagiwara, H; Ishido, M; Fujita, T; Kume, T; Ishigaki, I; Hirose, S

The interaction between the receptor (Rc) for atrial natriuretic peptide (ANP) and the effector enzyme particulate guanylate cyclase (GC) has been studied by radiation inactivation. Irradiation of bovine lung membranes produced an increase in GC activity at low radiation doses followed by a dose-dependent reduction at higher doses. This deviation from linearity in the inactivation curve disappeared when lung membranes were pretreated with ANP. Essentially identical results were also obtained with adrenal membranes. Based on these radiation inactivation data, the following dissociative mechanism of activation of particulate guanylate cyclase by ANP has been proposed: Rc.GC(inactive) + ANP----Rc.ANP + GC(active).

• Research Organization: Tokyo Institute of Technology (Japan)
• OSTI ID: 6462345
• Journal Information: Biochem. Biophys. Res. Commun.; (United States), Vol. 158:2
• Country of Publication: United States
• Language: English

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