Reaction center and light-harvesting I genes from Rhodopseudomonas capsulatta

Journal Article · Sun Jan 01 04:00:00 EST 1984 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.81.1.189· OSTI ID:6055401

Youvan, D C; Alberti, M; Begusch, H; Bylina, E J; Hearst, J E

Five structural genes coding for the reaction center (RC) L, M, and H subunits and the two light-harvesting (LH) I polypeptides, B870..cap alpha.. and B870..beta.., have been mapped on two restriction fragments from the R-prime plasmid pRPS404. It has been recently shown that enhanced near-infrared fluorescence mutants of Rhodopseudomonas capsulata typically lack RC or LH I polypeptides and that these lesions are marker-rescued by two restriction fragments from the R-prime plasmid: the 7.5-kilobase-pair EcoRI F fragment and the 4.75-kilobase-pair BamHI C-EcoRI fragment. We have now determined the nucleotide sequence of two restriction fragments and have found that the BamHI C-EcoRI B fragment carries the structural genes for the RC L and M subunits and both LH I polypeptides. Forty kilobase pairs away from this locus, the BamHI F fragment (within the EcoRI F fragment) carries the RC H subunit. The structural genes on the BamHI C-EcoRI B fragment are probably transcribed as part of a polycistronic mRNA. All of the structural genes begin with a consensus Shine-Dalgarno sequence and separate AUG start codons, indicating that the structural polypeptides are not cleaved from larger precursor polypeptides. 12 references, 4 figures.

• Research Organization: Univ. of California, Berkeley
• DOE Contract Number: AC03-76SF00098
• OSTI ID: 6055401
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 81:1; ISSN PNASA
• Country of Publication: United States
• Language: English

Related Subjects

550700* -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
CELL CONSTITUENTS
DATA
DNA SEQUENCING
EXPERIMENTAL DATA
GENETIC MAPPING
INFORMATION
MAPPING
MUTANTS
NUCLEOTIDES
NUMERICAL DATA
ORGANIC COMPOUNDS
PHOTOSYNTHETIC BACTERIA
PHOTOSYNTHETIC REACTION CENTERS
PLASMIDS
STRUCTURAL CHEMICAL ANALYSIS