Immunological comparison of the in vitro and in vivo labeled victorin binding protein from susceptible oats. [Avena sativa L]

Journal Article · Fri Mar 01 04:00:00 EST 1991 · Plant Physiology; (United States)

DOI:https://doi.org/10.1104/pp.95.3.917· OSTI ID:6053974

Wolpert, T J; Macko, V ^[1]

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1. Cornell Univ., Ithaca, NY (United States)

The fungus Cochliobolus victoriae causes victoria blight of oats and produces the host-specific toxin victorin. The reaction of oats to the fungus and its toxin is controlled by a single dominant gene whose product has been hypothesized to function as the site of action (receptor) of the toxin in susceptible oat genotypes. Previously, using a biologically active {sup 125}I derivative of the toxin, the authors identified a 100 kilodalton victorin-binding protein (VBP) which binds victorin in a ligand-specific manner and binds in vivo only in susceptible oat genotypes. However, a VBP in both the susceptible and resistant oat genotypes was identified by in vitro binding experiments. One interpretation of the lack of genotype-specific binding in vitro is that the 100 kilodalton protein detected in vitro is not the same 100 kilodalton protein detected in vivo. To clarify the relationship between the 100 kilodalton protein(s) labeled in vivo and in vitro, they developed antisera to the in vitro-labeled VBP from the susceptible genotype and demonstrated that these preparations react with the in vivo-labeled VBP from the susceptible genotype. This finding coupled with previous observations strongly suggest that the VBP observed in vivo is the same protein detected in vitro. Furthermore, the results support our previous observations which suggest that the VBPs labeled in vitro in susceptible and resistant genotypes are closely related or identical.

• OSTI ID: 6053974
• Journal Information: Plant Physiology; (United States), Journal Name: Plant Physiology; (United States) Vol. 95:3; ISSN 0032-0889; ISSN PLPHA
• Country of Publication: United States
• Language: English

Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANTIGENS
BETA DECAY RADIOISOTOPES
CEREALS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
GENES
GENOTYPE
GRAMINEAE
HALOGEN COMPOUNDS
IMMUNOLOGY
IN VITRO
IN VIVO
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IODINE 125
IODINE COMPOUNDS
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LILIOPSIDA
MAGNOLIOPHYTA
MATERIALS
MEMBRANE PROTEINS
NUCLEI
OATS
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PATHOGENS
PLANT DISEASES
PLANTS
PROTEINS
RADIOISOTOPES
RECEPTORS
TOXIC MATERIALS
TOXINS
TRACER TECHNIQUES