Antibody responses in allogeneic radiation chimeras

Thesis/Dissertation · Fri Jan 01 04:00:00 EST 1982

OSTI ID:6047971

Coico, R F

The construction of long-lived allogeneic radiation chimeras, free of graft-versus-host disease, has been achieved using serologic elimination of Thy 1/sup +/ cells from donor bone marrow. Humoral immune function was not restored in these animals as evidenced by lack of primary antibody responses to a T cell-dependent antigen, namely, sheep erythrocytes (SRBC) both in vivo and in vitro. No evidence for a suppressor cell-mediated mechanism was found. Using separated chimera spleen cell populations and specific helper cell soluble mediators, the functional capabilities of chimera B cells, T cells, and macrophages were assessed. These findings suggested that the failure of chimeras to produce antibody is not the result of impaired B cell, T cell, or macrophage function, but rather, that it is due to ineffective cellular interactions. Physiologic cellular interactions depend upon the sharing of major histocompatibility complex (MHC) determinants between interacting cells. However, the self-recognition repertoire of developing T cells may be influenced by the environment which these cells differentiate such that they learn to recognize host MHC determinants as self. These findings support the interpretation that the immunologic hyporeactivity of allogeneic bone marrow chimeras reflects the role of the host environment in restricting the interactive capabilities of donor-derived cells.

• Research Organization: New York Univ., NY (USA)
• OSTI ID: 6047971
• Country of Publication: United States
• Language: English

Related Subjects

551000 -- Physiological Systems
560152* -- Radiation Effects on Animals-- Animals
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMAL TISSUES
ANTIBODY FORMATION
ANTIGENS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
BONE MARROW
CHIMERAS
CONNECTIVE TISSUE CELLS
ERYTHROCYTES
HEMATOPOIETIC SYSTEM
IMMUNOLOGY
LEUKOCYTES
LYMPHOCYTES
MACROPHAGES
MATERIALS
MOSAICISM
ORGANS
PHAGOCYTES
RADIATION CHIMERAS
RADIOINDUCTION
SOMATIC CELLS
SPLEEN CELLS
TISSUES