Metabolism of the polychlorinated biphenyls (PCBs), Aroclor 1254, by the fungus Fusarium oxysporum
This study aimed at investigating the role of the fungus Fusarium oxysporum, often associated with polluted aquatic ecosystem, in metabolizing the PCBs Aroclor 1254. The results indicated the ability of the fungus in mineralizing Aroclor 1254 into CO/sub 2/ + H/sub 2/O at a rate of 0.053%-0.111% in seven days. PCBs with five or more chlorine atoms were incorporated into the fungal biomass by 48 hours after incubation. Lower chlorinated isomers were preferentially degraded after seven days of incubation. The presence of 1 gl/sup -1/D-dextose enhanced PCB degradation. Total soluble protein concentration was 24% below the controls after seven days of incubation in the presence of 5 mg l/sup -1/ Aroclor 1254. Protein analysis by 10% SDS-PAGE indicated the presence of Cytochrome P-450 like proteins at concentrations 40% above the control at 24 hours decreasing to levels below the controls by seven days. In contrast, Cytochrome P-448 like proteins gradually increased to 692% of the controls by seven days. Changes in the electrophoretic patterns and concentration of many proteins were also evident after treatment with Aroclor 1254.
- OSTI ID:
- 6047216
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560304* -- Chemicals Metabolism & Toxicology-- Invertebrates-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AQUATIC ECOSYSTEMS
AROMATICS
BIOLOGICAL ACCUMULATION
CHLORINATED AROMATIC HYDROCARBONS
CHROMATOGRAPHY
ECOSYSTEMS
ELECTROPHORESIS
FUNGI
FUSARIUM
HALOGENATED AROMATIC HYDROCARBONS
METABOLISM
ORGANIC CHLORINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
PARASITES
PHYSIOLOGY
PLANTS
PROTEINS
SEPARATION PROCESSES
THIN-LAYER CHROMATOGRAPHY