Restriction analysis of specific DNA sequences of five homothallic species of Neurospora

Journal Article · Sat Jan 01 04:00:00 EST 1983 · Genetics; (United States)

OSTI ID:6044778

Attoh, G; Dutta, S K

Nuclear DNA's from five homothallic species of Neurospora: N. africana, N. dodgei, N. lineolata, N. galapagosensis and N. terricola were isolated and characterized. From these total nuclear DNA's, specific DNA sequences were isolated by CsCl buoyant density gradient equilibrium centrifugation. These selected DNA's were restricted separately with EcoR1, Bam H1, Hind III, XBa 1 and Hinc II. DNA digests were electrophorused in varying agarose gel concentration (0.7%, 0.8% and 1%) and the sizes of fragments generated with these endonucleases were estimated against lambda phage DNA and pMF2 DNA fragments. EcoR1 generated four DNA size variants of about 20.0 kb, 7.5 kb, 5.5 b and 5.23 kb in N. terricola except the 10.5 kb fragment. Instead, N. lineolata showed three distinct DNA size variants of about 15.0 kb, 12.0 kb and 8.2 kb with Bam H1 treatment. Similarly we have noticed unique DNA restriction bands when treated with a few other restriction enzymes like Xba 1 and Hind III. These results suggest distinct rDNA nucleotide sequence differences among homothallic species of Neurospora studied.

• Research Organization: Howard Univ., Washington, DC
• OSTI ID: 6044778
• Journal Information: Genetics; (United States), Journal Name: Genetics; (United States) Vol. 104:1; ISSN GENTA
• Country of Publication: United States
• Language: English

Related Subjects

550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
BIOLOGICAL VARIABILITY
DNA
DNA SEQUENCING
DNA-ASE
ELECTROPHORESIS
ENDONUCLEASES
ENZYMES
ESTERASES
FUNGI
HYDROLASES
NEUROSPORA
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PHOSPHODIESTERASES
PLANTS
SIZE
STRUCTURAL CHEMICAL ANALYSIS