DNA homologies of ribosomal RNA genes of Neurospora species
Journal Article
·
· Neurospora Newsl.; (United States)
OSTI ID:5632158
Ribosomal RNA genes (rDNAs) of Neurospora crassa contain DNA sequences which code for 17S, 5.8S, and 26S rRNAs, in addition to internal and external spacers. As has been reported for many eukaryotes, the DNA sequences which code for 17S, 5.8S, and 26S rRNAs in Neurospora species are probably conserved while the internal and external spacer regions are probably variable sequences. Extensive electron microscopic studies of 45S precursor rRNA of several cold and warm blooded animals confirm that spacer regions vary extensively from species to species. It was desirable to know whether such differences in rDNA sequences exist between Neurospora species. Any such difference should be detectable using standard procedures for DNA homology studies rDNA sequences were isolated from N. crassa mycelial cells using the procedure described previously. The purified rDNA was /sup 3/H-labeled (by nick translation) and reassociated with total DNA isolated from the heterothallic species N. crassa and from three homothalliospecies: N. dodgei, N. lineolata, and N. africana. In addition, /sup 32/P-labeled total DNA of N. crassa was reannealed with unlabeled bulk DNA from N. crassa, N. dodgei, and N. lineolata.
- OSTI ID:
- 5632158
- Journal Information:
- Neurospora Newsl.; (United States), Journal Name: Neurospora Newsl.; (United States) Vol. 27; ISSN NEUNA
- Country of Publication:
- United States
- Language:
- English
Similar Records
Polymorphism in the ribosomal RNA genes in three heterothallic species of Neurospora
Differential gene expression in Neurospora crassa cell types: heterogeneity and multiple copies of rRNA genes. Annual progress report, July 1981-June 1982
Heterothallic species of neurospora are distinguishable by restriction analysis of their nuclear rDNA sequences
Journal Article
·
Sat Dec 31 23:00:00 EST 1983
· Genetics; (United States)
·
OSTI ID:7065548
Differential gene expression in Neurospora crassa cell types: heterogeneity and multiple copies of rRNA genes. Annual progress report, July 1981-June 1982
Technical Report
·
Thu Dec 31 23:00:00 EST 1981
·
OSTI ID:5177105
Heterothallic species of neurospora are distinguishable by restriction analysis of their nuclear rDNA sequences
Journal Article
·
Fri Dec 31 23:00:00 EST 1982
· Neurospora Newsl.; (United States)
·
OSTI ID:5910369
Related Subjects
550201* -- Biochemistry-- Tracer Techniques
550401 -- Genetics-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMISTRY
CELL CONSTITUENTS
CHEMISTRY
DAYS LIVING RADIOISOTOPES
DNA
FUNGI
GENES
GENETIC MAPPING
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
MAPPING
NEUROSPORA
NUCLEI
NUCLEIC ACIDS
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANOIDS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PLANTS
RADIOISOTOPES
RIBOSOMES
RNA
STRUCTURAL CHEMICAL ANALYSIS
TRITIUM COMPOUNDS
550401 -- Genetics-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMISTRY
CELL CONSTITUENTS
CHEMISTRY
DAYS LIVING RADIOISOTOPES
DNA
FUNGI
GENES
GENETIC MAPPING
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
MAPPING
NEUROSPORA
NUCLEI
NUCLEIC ACIDS
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANOIDS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PLANTS
RADIOISOTOPES
RIBOSOMES
RNA
STRUCTURAL CHEMICAL ANALYSIS
TRITIUM COMPOUNDS