Heterothallic species of neurospora are distinguishable by restriction analysis of their nuclear rDNA sequences

Journal Article · Sat Jan 01 04:00:00 EST 1983 · Neurospora Newsl.; (United States)

OSTI ID:5910369

Chambers, C; Dutta, S K

Restriction analysis of rDNAs was used to distinguish nuclear rDNA's of three different reference strains of heterothallic species of the genus Neurospora: N. crassa 74A (FGSC number987), N. intermedia P420 (FGSC number2316), and N. sitophila 10B (FGSC number580). Two approaches were adopted: (1) Nuclear DNA's of these three Neurospora species were treated with various restriction enzymes. Against the streaks of nuclear DNAs on the 0.7% agarose gels background bands were visible. These background bands are visible because rDNA sequences of Neurospora species exist in multiple copies within the nuclear DNA's. (2) The second approach was comparison of auto-radiographs of hybrid molecules of Southern blot transfers of restricted nuclear DNAs and /sup 32/P-labelled nick translated rDNA's (referred to as rDNA probe) isolated from N. crassa slime mutant (FGSC number1118), rDNA cloned into pBR322. A summary of restricted fragment sizes as seen in the gels and in autoradiographs of Southern blots of the respective gels is presented.

• OSTI ID: 5910369
• Journal Information: Neurospora Newsl.; (United States), Journal Name: Neurospora Newsl.; (United States) Vol. 30; ISSN NEUNA
• Country of Publication: United States
• Language: English

Related Subjects

550401* -- Genetics-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL VARIABILITY
BIOLOGY
DAYS LIVING RADIOISOTOPES
DNA
DNA SEQUENCING
ELECTROPHORESIS
ENZYMES
FUNGI
GENETIC VARIABILITY
GENETICS
HYBRIDIZATION
ISOTOPES
LIGHT NUCLEI
NEUROSPORA
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PLANTS
RADIOISOTOPES
STRUCTURAL CHEMICAL ANALYSIS