Direct effect of carbon monoxide on hexobarbital metabolism in the isolated perfused liver in the absence of hemoglobin

Journal Article · Tue Jan 01 00:00:00 EST 1985 · J. Toxicol. Environ. Health; (United States)

DOI:https://doi.org/10.1080/15287398509530710· OSTI ID:6037405

Takano, T; Motohashi, Y; Miyazaki, Y; Okeda, R

The interaction of carbon monoxide (CO) with cytochrome P-450 associated with hexobarbital metabolism was observed in hemoglobin-free perfused rat liver by using a scanning reflectance spectrophotometer. The evidence obtained showed that CO bound to the substrate complexed cytochrome P-450 and, at a CO/O2 ratio of over 0.1 in the perfusate, inhibited the hexobarbital metabolism estimated from the hexobarbital uptake, and oxygen consumption. Although the oxygen supply to the liver cell was one of the major limiting factors during CO hypoxia, CO binding to cytochrome P-450 significantly enhanced the suppression of hexobarbital oxidation caused by hypoxic hypoxia.

• Research Organization: Tokyo Medical and Dental Univ., Japan
• OSTI ID: 6037405
• Journal Information: J. Toxicol. Environ. Health; (United States), Vol. 15:6
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BARBITURATES
METABOLISM
CARBON MONOXIDE
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
OXIDOREDUCTASES
ANOXIA
HEMOGLOBIN
INHIBITION
LIVER
PERFUSED ORGANS
RATS
SPECTROPHOTOMETRY
ANESTHETICS
ANIMALS
AZINES
BODY
CARBON COMPOUNDS
CARBON OXIDES
CARBOXYLIC ACIDS
CENTRAL NERVOUS SYSTEM DEPRESSANTS
CHALCOGENIDES
DIGESTIVE SYSTEM
DRUGS
ENZYMES
GLANDS
GLOBIN
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYPNOTICS AND SEDATIVES
KINETICS
MAMMALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
ORGANS
OXIDES
OXYGEN COMPOUNDS
PIGMENTS
PORPHYRINS
PROTEINS
PYRIMIDINES
REACTION KINETICS
RODENTS
VERTEBRATES
560305* - Chemicals Metabolism & Toxicology- Vertebrates- (-1987)