Effect of carbon monoxide on the cytochrome P-450-mediated metabolism of aniline and p-nitroanisole in the isolated perfused rabbit lung

Journal Article · Sun Jan 01 04:00:00 EST 1989 · Journal of Toxicology and Environmental Health; (USA)

DOI:https://doi.org/10.1080/15287398909531304· OSTI ID:5078660

Trela, B A; Carlson, G P ^[1]; Mayer, P R ^[1]

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1. Purdue Univ., West Lafayette, IN (USA)

Carbon monoxide (CO), an environmental pollutant, inhibits the cytochrome P-450-mediated metabolism of xenobiotics in vitro. In recent years, the importance of the lung in the metabolic disposition of certain airborne and systemically administered xenbiotics has been demonstrated. The purpose of this investigation was to establish a threshold for the CO-induced inhibition of cytochrome P-450-mediated activities in the isolated perfused rabbit lung and to determine if these reactions are equally sensitive to this toxicant in this model. Neither the mixed-function oxidase-mediated hydroxylation nor the acetylation of aniline was altered by exposure to 7.5% CO/20% O{sub 2} for 2.5 h in the isolated perfused rabbit lung. p-Nitroanisole O-demethylation by isolated rabbit lungs ventilated with 7.5% CO/20% O{sub 2} was significantly decreased ({approximately} 37%) in comparison to control. That these reactions are not similarly influenced by carbon monoxide may indicate that the constitutive isozymes of cytochrome P-450 in the rabbit lung are differentially sensitive to CO-induced inhibition.

• OSTI ID: 5078660
• Journal Information: Journal of Toxicology and Environmental Health; (USA), Journal Name: Journal of Toxicology and Environmental Health; (USA) Vol. 27:3; ISSN JTEHD; ISSN 0098-4108
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINES
ANILINE
ANIMALS
AROMATICS
BODY
CARBON COMPOUNDS
CARBON MONOXIDE
CARBON OXIDES
CHALCOGENIDES
ENZYME ACTIVITY
ENZYMES
INHIBITION
LUNGS
MAMMALS
METABOLISM
MIXED-FUNCTION OXIDASES
NITRO COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
OXIDES
OXIDOREDUCTASES
OXYGEN COMPOUNDS
OXYGENASES
PERFUSED ORGANS
RABBITS
RESPIRATORY SYSTEM
TOXICITY
VERTEBRATES