Dried Staphylococcus aureus as a rapid immunological separating agent in radioimmunoassays

Journal Article · Thu Feb 01 04:00:00 EST 1979 · J. Clin. Endocrinol. Metab.; (United States)

DOI:https://doi.org/10.1210/jcem-48-2-360· OSTI ID:6037157

Ying, S Y; Guillemin, R

A rapid (less than or equal to 60 seconds) immunological separation of antigen-antibody complexes from free antigen has been developed in radioimmunoassays (RIAs) of luteinizing hormone (LH), follicle stimulating hormone (FSH), prolactin (PRL) and ..beta..-endorphin by using suspensions of dried Staphylococcus aureus rich in protein-A. In the systems tested parallel dose-response curves were obtained for protein-A and second antibody precipitations. The sensitivity of the protein-A method is equal to or higher than that of second antibody method. Tissue culture medium and serum hormone levels measured with RIAs using protein-A are similar to those detected with double antibody methods. The technique may be of general use in all RIAs utilizing antisera from species whose IgG are known to be bound by protein-A.

• Research Organization: Salk Inst. for Biological Studies, La Jolla, CA
• OSTI ID: 6037157
• Journal Information: J. Clin. Endocrinol. Metab.; (United States), Journal Name: J. Clin. Endocrinol. Metab.; (United States) Vol. 48:2; ISSN JCEMA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ANTIBODIES
ANTIGENS
BACTERIA
DATA
DATA FORMS
ENKEPHALINS
EXPERIMENTAL DATA
FSH
GONADOTROPINS
GRAPHS
HORMONES
INFORMATION
ISOLATED VALUES
ISOTOPE APPLICATIONS
LH
LTH
MICROORGANISMS
NUMERICAL DATA
ORGANIC COMPOUNDS
PEPTIDE HORMONES
PEPTIDES
PITUITARY HORMONES
POLYPEPTIDES
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
STAPHYLOCOCCUS
TRACER TECHNIQUES