Conserved nodulation genes in Rhizobium meliloti and Rhizobium trifolii

Journal Article · Sat Jun 01 04:00:00 EDT 1985 · Appl. Environ. Microbiol.; (United States)

OSTI ID:6035564

Fisher, R F; Tu, J K; Long, S R

Plasmids which contained wild-type or mutated Rhizobium meliloti nodulation (Nod) genes were introduced into Nod/sup -/ R. trifolii mutants ANU453 and ANU851 and tested for their ability to nodulate clover. Cloned wild-type and mutated R. meliloti Nod gene segments restored ANU851 to Nod/sup +/, with the exception of nodD mutants. Similarly, wild-type and mutant R. meliloti nod genes complemented ANU453 to Nod/sup +/, except for nod CII mutants. Thus, ANU851 identifies the equivalent of the R. meliloti nodD genes, and ANU453 specifies the equivalent of the R. meliloti nodCII genes. In addition, cloned wild-type R. trifolii nod genes were introduced into seven R. meliloti Nod/sup -/ mutants. All seven mutants were restored to Nod/sup +/ on alfalfa. Our results indicate that these genes represent common nodulation functions and argue for an allelic relationship between nod genes in R. meliloti and R. trifolii.

• Research Organization: Stanford Univ., CA
• DOE Contract Number: AT03-82ER12084
• OSTI ID: 6035564
• Journal Information: Appl. Environ. Microbiol.; (United States), Journal Name: Appl. Environ. Microbiol.; (United States) Vol. 49:6; ISSN AEMID
• Country of Publication: United States
• Language: English

Related Subjects

550400 -- Genetics
550700* -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
BACTERIA
CELL CONSTITUENTS
CLONING
DNA-CLONING
GENES
GENETIC ENGINEERING
MICROORGANISMS
MUTANTS
NITROGEN FIXATION
PLASMIDS
RHIZOBIUM