Extended region of nodulation genes in Rhizobium meliloti 1021. I. Phenotypes of Tn5 insertion mutants

Journal Article · Thu Oct 01 04:00:00 EDT 1987 · Genetics; (United States)

OSTI ID:5355643

Swanson, J A; Tu, J K; Ogawa, J; Sanga, R; Fisher, R F; Long, S R

Rhizobium meliloti Nod/sup -/ mutant WL131, a derivative of wild-type strain 102F51, was complemented by a clone bank of wild-type R. meliloti 1021 DNA, and clone pRmJT5 was recovered. Transfer of pRmJT5 conferred alfalfa nodulation on other Rhizobium species, indicating a role in host range determination for pRmJT5. Mutagenesis of pRmJT5 revealed several segments in which transposon insertion causes delay in nodulation, and/or marked reduction of the number of nodules formed on host alfalfa plants. The set of mutants indicated five regions in which nod genes are located; one mutant, nod-216, is located in a region not previously reported to encode a nodulation gene. Other mutant phenotypes correlated with the positions of open reading frames for nodH, nodF and nodE, and with a 2.2-kb EcoRI fragment. A mutant in nodG had no altered phenotype in this strain. One nodulation mutant was shown to be a large deletion of the common nod gene region. The authors present a discussion comparing the various studies made on this extended nod gene region.

• Research Organization: Stanford Univ., CA
• DOE Contract Number: AT03-82ER12084
• OSTI ID: 5355643
• Journal Information: Genetics; (United States), Journal Name: Genetics; (United States) Vol. 117:2; ISSN GENTA
• Country of Publication: United States
• Language: English

Related Subjects

550401* -- Genetics-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AUTORADIOGRAPHY
BACTERIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CELL CONSTITUENTS
CLONING
DAYS LIVING RADIOISOTOPES
DNA
DNA-CLONING
GENETIC MAPPING
HYBRIDIZATION
ISOTOPES
LIGHT NUCLEI
MAPPING
MICROORGANISMS
MUTANTS
MUTATIONS
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHENOTYPE
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PLASMIDS
RADIOISOTOPES
RHIZOBIUM