Kinetics of accumulation and processing of simian virus 40 RNA in Xenopus laevis oocytes injected with simian virus 40 DNA

Journal Article · Wed Dec 01 04:00:00 EST 1982 · Mol. Cell. Biol.; (United States)

DOI:https://doi.org/10.1128/MCB.2.12.1581· OSTI ID:6030621

Miller, T J; Stephens, D L; Mertz, J E

The authors examined the kinetics of accumulation and processing of simian virus 40 (SV40) RNA in stage 6 oocytes of Xenopus laevis microinjected intranuclearly with SV40 DNA. The rates of synthesis and degradation, cellular distribution, size, and sequence specificity of radiolabelled SV40-specific and endogeneous oocyte RNA were determined. The kinetics of accumulation of SV40 RNA were biphasic, with greater than 90% of the viral RNA turning over in the nucleus with a half-life of 20 to 40 min. Although most of the primary transcription products were mulitgenomic in length, some table polyadenylated SV40-specific RNA similar in size and sequence to late 19S mRNA accumulated in the cytoplasm with time. Differences in strand preference, efficiencies of transcription termination and polyadenylation, and the splice sites used in synthesis and processing of SV40 RNA in Xenopus oocytes and monkey cells were noted. However, these differences were quantitative, rather than qualitative, in nature. Consequently, they are probably due to regulatory rather than mechanistic differences between the two cell types. The authors therefore conclude that Xenopus oocytes may be a useful system for studying both mechanistic and cell type-specific regulatory aspects of mRNA biogenesis from cloned DNAs. However, since only a small percentage of the initially synthesized RNA ends up in stable mRNA, it will be important to determine whether mutants of cloned DNAs that produce abnormal amounts of stable mRNAs are altered in promotion and initiation of RNA synthesis, transcription termination, RNA processing, or the stability of the resultant mRNAs.

• Research Organization: McArdle Lab. for Cancer Research, Univ. of Wisconsin, Madison, WI 53706
• OSTI ID: 6030621
• Journal Information: Mol. Cell. Biol.; (United States), Journal Name: Mol. Cell. Biol.; (United States) Vol. 2:12; ISSN MCEBD
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
550400 -- Genetics
59 BASIC BIOLOGICAL SCIENCES
ADENYLIC ACID
AMPHIBIANS
ANIMALS
AQUATIC ORGANISMS
BACTERIOPHAGES
BIOCHEMICAL REACTION KINETICS
BIOSYNTHESIS
CELL CULTURES
CLONE CELLS
DNA
FROGS
GAMETES
GERM CELLS
HALF-LIFE
ISOTOPE APPLICATIONS
KINETICS
LABELLED POOL TECHNIQUES
MESSENGER-RNA
METABOLISM
MICROORGANISMS
MUTANTS
NUCLEIC ACIDS
NUCLEOTIDES
ORGANIC COMPOUNDS
OVA
PARASITES
PROTEINS
REACTION KINETICS
RECOMBINANT DNA
RNA
RNA PROCESSING
SIMIAN VIRUS
SIZE
SPECIFICITY
SYNTHESIS
TRACER TECHNIQUES
TRANSCRIPTION
VERTEBRATES
VIRUSES