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Regulatory effects of eicosanoids on thymidine uptake by vascular smooth muscle cells of rats

Journal Article · · Prostaglandins; (United States)
; ; ; ; ;

To define the roles of eicosanoids in vascular smooth muscle cells (VSMC) growth, we examined the effects of exogenous eicosanoids on (/sup 3/H)thymidine uptake by cultured VSMC of Wistar rats. Stable prostacyclin (PGI2) analog, OP-41483, significantly decreased the incorporation of (/sup 3/H)thymidine into deoxyribonucleic acid (DNA) of VSMC in a dose dependent manner from 10(-8) to 10(-4) M. Prostaglandin E2 (PGE2) and PGD2 ranging from 10(-8) to 10(-4) M also dose-dependently decreased the (/sup 3/H)thymidine uptake by VSMC. In contrast, stable thromboxane A2 analog, STA2, significantly increased the incorporation of (/sup 3/H)thymidine into DNA in a dose dependent manner from 10(-8) to 10(-4) M. The dose response curve of STA2 was shifted toward a lowered response when 10(-5) M PGI2 analog, PGE2 or PGD2 was added in the culture medium. Thus, it is indicated that vasodepressor eicosanoids decrease the proliferation of VSMC, whereas vasoconstrictor TXA2 enhances the VSMC growth. Vascular smooth muscle cells possibly autoregulate the cell proliferation through the eicosanoids generation.

Research Organization:
Univ. of Tokyo (Japan)
OSTI ID:
6030493
Journal Information:
Prostaglandins; (United States), Journal Name: Prostaglandins; (United States) Vol. 36:6; ISSN PRGLB
Country of Publication:
United States
Language:
English

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Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
AZINES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL FUNCTIONS
CARBOXYLIC ACIDS
CARDIOVASCULAR AGENTS
CELL PROLIFERATION
DNA
DOSE-RESPONSE RELATIONSHIPS
DRUGS
EICOSANOIC ACID
FUNCTIONS
HETEROCYCLIC COMPOUNDS
IMMUNOASSAY
IMMUNOLOGY
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
MAMMALS
MONOCARBOXYLIC ACIDS
MUSCLES
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PROSTAGLANDINS
PYRIMIDINES
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
RATS
REACTION KINETICS
RIBOSIDES
RODENTS
THYMIDINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
UPTAKE
VASOCONSTRICTORS
VERTEBRATES