Epidermal growth factor-mediated effects on equine vascular smooth muscle cells
Journal Article
·
· American Journal of Physiology; (USA)
OSTI ID:6254178
- Texas A M Univ., College Station (USA)
Epidermal growth factor (EGF) receptor binding kinetics and EGF-mediated stimulation of DNA synthesis and cellular proliferation were studied in cultured vascular smooth muscle cells (VSMC) from the equine thoracic aorta. Binding studies, using murine {sup 125}I-labeled EGF, indicate the presence of a single class of high-affinity binding sites, with an estimated maximal binding capacity of 5,800 sites/cells. EGF stimulated ({sup 3}H)thymidine uptake in confluent quiescent monolayers in a dose-dependent fashion, half-maximal stimulation occurring at 7.5 {times} 10{sup {minus}11} M. Likewise, EGF-mediated cellular proliferation was dose dependent under reduced serum concentrations. Equine VSMC contain specific receptors for EGF, and EGF can stimulate DNA synthesis and proliferation in these cultured cells, which suggests that EGF may participate in the proliferative changes observed in equine distal digital peripheral vascular disease.
- OSTI ID:
- 6254178
- Journal Information:
- American Journal of Physiology; (USA), Journal Name: American Journal of Physiology; (USA) Vol. 255:4; ISSN 0002-9513; ISSN AJPHA
- Country of Publication:
- United States
- Language:
- English
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550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL TISSUES
ANIMALS
AZINES
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BODY
CELL PROLIFERATION
DAYS LIVING RADIOISOTOPES
DNA REPLICATION
DOSE-RESPONSE RELATIONSHIPS
ELECTRON CAPTURE RADIOISOTOPES
EPIDERMIS
EPITHELIUM
GROWTH FACTORS
HETEROCYCLIC COMPOUNDS
HORSES
HYDROGEN COMPOUNDS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPES
KINETICS
LABELLED COMPOUNDS
MAMMALS
MEMBRANE PROTEINS
MITOGENS
MUSCLES
NUCLEI
NUCLEIC ACID REPLICATION
NUCLEOSIDES
NUCLEOTIDES
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PROTEINS
PYRIMIDINES
RADIOISOTOPES
REACTION KINETICS
RECEPTORS
RIBOSIDES
SKIN
THYMIDINE
TISSUES
TRITIUM COMPOUNDS
UPTAKE
VERTEBRATES
59 BASIC BIOLOGICAL SCIENCES
ANIMAL TISSUES
ANIMALS
AZINES
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
BODY
CELL PROLIFERATION
DAYS LIVING RADIOISOTOPES
DNA REPLICATION
DOSE-RESPONSE RELATIONSHIPS
ELECTRON CAPTURE RADIOISOTOPES
EPIDERMIS
EPITHELIUM
GROWTH FACTORS
HETEROCYCLIC COMPOUNDS
HORSES
HYDROGEN COMPOUNDS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPES
KINETICS
LABELLED COMPOUNDS
MAMMALS
MEMBRANE PROTEINS
MITOGENS
MUSCLES
NUCLEI
NUCLEIC ACID REPLICATION
NUCLEOSIDES
NUCLEOTIDES
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PROTEINS
PYRIMIDINES
RADIOISOTOPES
REACTION KINETICS
RECEPTORS
RIBOSIDES
SKIN
THYMIDINE
TISSUES
TRITIUM COMPOUNDS
UPTAKE
VERTEBRATES