Phosphorylation of atrial natriuretic peptide prohormone
Previously they have shown that atrial natriuretic peptides (ANP) are excellent substrates for cAMP-dependent protein kinase. The site of in vitro phosphorylation occurs at Ser 104, and is contained in a typical recognition sequence for cAMP-dependent protein kinase, Arg 101-Arg 102-Ser 103-Ser 104. In this report the prohormone pro-ANP, the predominant form present in atrial secretory granules, was purified from rat atria. Like ANP, pro-ANP was also found to be phosphorylated by cAMP-dependent protein kinase in vitro. Peptide mapping studies carried out with in vitro-phosphorylated pro-ANP revealed predominantly one /sup 32/P-labeled peptide. This was demonstrated to be the same hexapeptide, Arg 101-Phe 106, found earlier for phosphorylated ANP. The amino acid sequence analysis also suggests that the site of phosphorylation is located at Ser 104. When isolated rat atria were incubated in the presence of /sup 32/P-orthophosphate, the pro-ANP purified from these atria was observed to be radioactive. The in situ incorporation of /sup 32/P into pro-ANP was confirmed by SDS polyacrylamide gel electrophoresis followed by immunoblotting and autoradiography.
- Research Organization:
- Univ. of Health Sciences/Chicago Medical School, Chicago, IL
- OSTI ID:
- 6028903
- Report Number(s):
- CONF-870644-
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Vol. 46:6; ISSN FEPRA
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CARDIOVASCULAR SYSTEM
CHEMICAL REACTIONS
DAYS LIVING RADIOISOTOPES
ENZYMES
HEART
HORMONES
IN VITRO
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
MAMMALS
MOLECULAR STRUCTURE
NUCLEI
ODD-ODD NUCLEI
ORGANS
PEPTIDE HORMONES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PHOSPHORUS-GROUP TRANSFERASES
PHOSPHORYLATION
PHOSPHOTRANSFERASES
RADIOISOTOPES
RATS
RODENTS
TRACER TECHNIQUES
TRANSFERASES
VERTEBRATES