Two dimensional gel electrophoretic resolution of the polypeptides of mitochondria isolated from different rat organs

Conference · Fri May 01 00:00:00 EDT 1987 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:6028381

Haldar, D; Osorno, H; Diaz, J; Lipfert, L; Sheehan, D

A comparative study of rat liver, kidney, heart, spleen, and brain mitochondria were made by resolving their polypeptides by two dimensional gel electrophoresis and staining with silver. The mitochondria were purified using either a sucrose or a ficoll gradient and the purity monitored by enzyme markers. Mitochondria from each organ exhibited a characteristic 2-D gel pattern. There were many similarities and differences between the mitochondria isolated from different organs. Under optimal conditions, the liver mitochondria (40 ..mu..g) produced 200 to 250 spots. The total number of spots decreased in the order: liver > brain > heart, kidney > spleen. The liver mitochondria showed no spots in the pI <5 and there was a predominant spot of carbamyl phosphate synthetase (CPS). The other mitochondrial preparations showed hardly any CPS but showed some other major spots. Identification of some of the membrane proteins were made by different methods, e.g., VDAC and MAO by parallel migration of the pure proteins, MAO by labeling with /sup 3/H-pargyline, and the alpha- and beta-subunits of the F/sub 1/ ATPase by comparing with published data.

• Research Organization: St. John's Univ., New York, NY
• OSTI ID: 6028381
• Report Number(s): CONF-870644-; TRN: 87-037203
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 46:6; Conference: 78. annual meeting of the American Society of Biological Chemists conference, Philadelphia, PA, USA, 7 Jun 1987
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
POLYPEPTIDES
ELECTROPHORESIS
BRAIN
COMPARATIVE EVALUATIONS
HEART
KIDNEYS
LIVER
RATS
SPLEEN
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ANIMALS
BODY
CARDIOVASCULAR SYSTEM
CENTRAL NERVOUS SYSTEM
DIGESTIVE SYSTEM
GLANDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
NERVOUS SYSTEM
ORGANIC COMPOUNDS
ORGANS
PEPTIDES
PROTEINS
RODENTS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques