Correlating survival and DNA excision repair in human fibroblasts
A model is presented that correlates the survival of cells in tissue culture exposed to toxic agents with the rate of excision of residues bound to DNA. Under the assumptions of the model, data on the survival of normal human fibroblasts (NF) and fibroblasts from xeroderma pigmentosum (XP) patients in complementation groups A, C and E (XPA, XPC and XPE) after exposure to N-acetoxy-2-acetylaminofluorene (N-AcO-AAF) were used to predict the rate of excision of N-AcO-AAF-induced DNA residues by the partially repair deficient cells relative to the rate of excision by NF. These predictions were tested by measurements of the rate of removal of radiolabeled N-AcO-AAF-induced DNA residues by fibroblasts in confluent cultures. In general, XPE removed N-AcO-AAF-induced DNA residues at the rate predicted by the model while the rate of repair by XPC was slower than predicted. The results with XPC indicate that this cell strain not only has a reduced rate of DNA excision repair but also differs from NF in other factors that affect cell survival. 21 references, 2 figures.
- Research Organization:
- Pacific Northwest Labs., Richland, WA (USA); National Center for Toxicological Research, Jefferson, AR (USA)
- DOE Contract Number:
- AC06-76RL01830
- OSTI ID:
- 6027708
- Report Number(s):
- PNL-SA-11304; ON: DE85008578
- Resource Relation:
- Other Information: Portions of this document are illegible in microfiche products
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
FIBROBLASTS
DNA REPAIR
SURVIVAL CURVES
BIOLOGICAL EFFECTS
CONDENSED AROMATICS
EXPERIMENTAL DATA
ORGANIC FLUORINE COMPOUNDS
TISSUE CULTURES
TOXIC MATERIALS
ANIMAL CELLS
AROMATICS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CONNECTIVE TISSUE CELLS
DATA
INFORMATION
MATERIALS
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
RECOVERY
REPAIR
SOMATIC CELLS
560301* - Chemicals Metabolism & Toxicology- Cells- (-1987)