Delineation of myocardial oxygen utilization with carbon-11-labeled acetate

Journal Article · Tue Sep 01 00:00:00 EDT 1987 · Circulation; (United States)

DOI:https://doi.org/10.1161/01.CIR.76.3.687· OSTI ID:6025865

Brown, M; Marshall, D R; Sobel, B E; Bergmann, S R

Although positron-emission tomography (PET) with labeled fatty acid delineates infarct size and permits qualitative assessment of fatty acid utilization, quantification of oxidative metabolism is limited by complex alterations in the pattern of utilization of fatty acid during ischemia and reperfusion. Because metabolism of acetate by myocardium is less complex than that of glucose or palmitate, we characterized kinetics of utilization of radiolabeled acetate in 37 isolated rabbit hearts perfused with modified Krebs-Henseleit buffer and performed a pilot tomographic study in man. Results of initial experiments with carbon-14-labeled acetate (/sup 14/C-acetate) indicated that the steady-state extraction fraction of acetate averaged 61.5 +/- 4.0% in control hearts (n = 4), 93.6 +/- 0.9% in hearts rendered ischemic (n = 4), and 54.8 +/- 4.0% in hearts reperfused after 60 min of ischemia (n = 3). Oxidation of /sup 14/C-acetate, assessed from the rate of efflux of /sup 14/CO/sub 2/ in the venous effluent, correlated closely with the rate of oxygen consumption under diverse metabolic conditions (r = .97, p less than .001). In addition, no significant differences were observed between rates of efflux of total /sup 14/C in all chemical species (reflecting total clearance of tracer from myocardium) and efflux of /sup 14/CO/sub 2/. Clearance of /sup 11/C-acetate, measured externally with gamma probes in normal and ischemic myocardium, correlated closely with clearance of /sup 14/C-acetate measured directly in the effluent (r = .99, p less than .001) and with overall myocardial oxygen consumption (r = .95, p less than .001). Accumulation and clearance of /sup 11/C-acetate from human myocardium with PET demonstrated kinetics comparable to those seen with radiolabeled acetate in vitro.

• Research Organization: Washington Univ. School of Medicine, St. Louis, MO
• OSTI ID: 6025865
• Journal Information: Circulation; (United States), Journal Name: Circulation; (United States) Vol. 76:3; ISSN CIRCA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ACETATES
ALDEHYDES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-PLUS DECAY RADIOISOTOPES
BIOLOGICAL HALF-LIFE
BODY
CARBOHYDRATES
CARBON 11
CARBON 14 COMPOUNDS
CARBON COMPOUNDS
CARBON DIOXIDE
CARBON ISOTOPES
CARBON OXIDES
CARBOXYLIC ACID SALTS
CARBOXYLIC ACIDS
CARDIOVASCULAR DISEASES
CARDIOVASCULAR SYSTEM
CHALCOGENIDES
COMPARATIVE EVALUATIONS
COMPUTERIZED TOMOGRAPHY
DIAGNOSIS
DIAGNOSTIC TECHNIQUES
DISEASES
ELEMENTS
EMISSION COMPUTED TOMOGRAPHY
EVEN-ODD NUCLEI
GLUCOSE
HEART
HEXOSES
ISCHEMIA
ISOTOPES
LABELLED COMPOUNDS
LACTATES
LIGHT NUCLEI
MAMMALS
METABOLISM
MINUTES LIVING RADIOISOTOPES
MONOSACCHARIDES
MUSCLES
MYOCARDIAL INFARCTION
MYOCARDIUM
NONMETALS
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
OXIDES
OXYGEN
OXYGEN COMPOUNDS
PERFUSED ORGANS
POSITRON COMPUTED TOMOGRAPHY
RABBITS
RADIOISOTOPES
SACCHARIDES
TOMOGRAPHY
VASCULAR DISEASES
VERTEBRATES