Mutagenic activity in diesel exhaust particulates
Journal Article
·
· Bull. Environ. Contam. Toxicol.; (United States)
Chloroform eluates of diesel exhaust particulate extracts were mutagenic in the Ames test. The mutagenic activity of approximately 480 revertants/100 ..mu..g of extract/plate did not require liver enzymes for its expression. The addition of liver enzymes decreased mutagenic activity, an effect which has previously been shown to be non-specific binding of the mutagen to liver proteins. After reduction of the diesel exhaust extract with sodium borohydride, the S-9 independent activity was greatly diminished. Addition of S-9 to the reduced sample revealed the presence of S-9 dependent mutagenic activity. These results, although not conclusive, provide additional evidence for the presence of nitronarene mutagens in diesel exhaust particulates. If, for example, the samples contained nitrofluorene, nitronaphthalene or nitropyrene, the reduction reaction would have formed aminofluorene, aminonaphthalene, and aminopyrene, compounds which are mutagens requiring liver enzyme activation in the Ames test. Attempts to isolate the amine by acidic extraction were not successful, however, because of the weak mutagenic activity in the sample.
- Research Organization:
- Univ. of California, Berkeley
- OSTI ID:
- 6025231
- Journal Information:
- Bull. Environ. Contam. Toxicol.; (United States), Journal Name: Bull. Environ. Contam. Toxicol.; (United States) Vol. 27:4; ISSN BECTA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
02 PETROLEUM
020600 -- Petroleum-- Health & Safety
020900 -- Petroleum-- Environmental Aspects
560302* -- Chemicals Metabolism & Toxicology-- Microorganisms-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKALI METAL COMPOUNDS
AROMATICS
BOROHYDRIDES
BORON COMPOUNDS
DIESEL ENGINES
DIESEL FUELS
ENGINES
ENVIRONMENTAL EFFECTS
EXHAUST GASES
EXTRACTION
FLUIDS
GASEOUS WASTES
GASES
HEAT ENGINES
HYDROGEN COMPOUNDS
INTERNAL COMBUSTION ENGINES
MUTAGEN SCREENING
MUTAGENESIS
NITRO COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PARTICLES
PARTICULATES
PETROLEUM PRODUCTS
SCREENING
SEPARATION PROCESSES
SODIUM COMPOUNDS
SOLVENT EXTRACTION
WASTES
020600 -- Petroleum-- Health & Safety
020900 -- Petroleum-- Environmental Aspects
560302* -- Chemicals Metabolism & Toxicology-- Microorganisms-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKALI METAL COMPOUNDS
AROMATICS
BOROHYDRIDES
BORON COMPOUNDS
DIESEL ENGINES
DIESEL FUELS
ENGINES
ENVIRONMENTAL EFFECTS
EXHAUST GASES
EXTRACTION
FLUIDS
GASEOUS WASTES
GASES
HEAT ENGINES
HYDROGEN COMPOUNDS
INTERNAL COMBUSTION ENGINES
MUTAGEN SCREENING
MUTAGENESIS
NITRO COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PARTICLES
PARTICULATES
PETROLEUM PRODUCTS
SCREENING
SEPARATION PROCESSES
SODIUM COMPOUNDS
SOLVENT EXTRACTION
WASTES