Biochemical studies of the differentiation of HL-60 cells into monocytes by either IFN, VIT, D/sub 3/ or TPA
The authors have studied the differentiation process of the human promyelocytic cell line, HL-60, by treatment of these cells with either gamma interferon, 1, 25 dihydroxyvitamin D/sub 3/ or a phorbol ester, TPA. The cells were grown in RPMI 1640, 10% FCS with each respective agent, then pulsed labeled with /sup 35/S-Met, harvested, lysed and subfractionated by centrifugation into post-ribosomal and ribosomal salt was fractions (RSW). These fractions were examined by SDS gel electrophoresis. The culture supernatant from the treated cells was dialyzed and passed over a heparin agarose affinity column. The absorbed material was eluted from the column by a step-wise salt gradient and analyzed by SDS gel electrophoresis. They have also observed that in a rabbit reticulocyte lysate assay, the RSW from control cells show inhibition of protein synthesis. The RSW from cells treated with either high concentrations (200-1000 units/ml) of gamma interferon, Vit D/sub 3/ or TPA did not show this inhibition. Some possible explanations for this phenomenon are the loss or inactivation of a component necessary for protein synthesis which is triggered by differentiation, or the differentiation-related modulation of translational inhibitor(s). They have used FPLC to further analyze the RSW, but because the factor(s) are present in such small quantities further analytical and more sensitive procedures need to be pursued.
- Research Organization:
- New York Medical College, Valhalla
- OSTI ID:
- 6015765
- Report Number(s):
- CONF-870644-; TRN: 87-037626
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 46:6; Conference: 78. annual meeting of the American Society of Biological Chemists conference, Philadelphia, PA, USA, 7 Jun 1987
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
CELL DIFFERENTIATION
INTERFERON
BIOLOGICAL EFFECTS
PHORBOL ESTERS
VITAMIN D
BIOCHEMISTRY
CENTRIFUGATION
ELECTROPHORESIS
INHIBITION
LIQUID COLUMN CHROMATOGRAPHY
METHIONINE
MONOCYTES
SULFUR 35
TRACER TECHNIQUES
AMINO ACIDS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOXYLIC ACIDS
CARCINOGENS
CHEMISTRY
CHROMATOGRAPHY
DAYS LIVING RADIOISOTOPES
DRUGS
ESTERS
EVEN-ODD NUCLEI
GROWTH FACTORS
ISOTOPE APPLICATIONS
ISOTOPES
LEUKOCYTES
LIGHT NUCLEI
LIPOTROPIC FACTORS
LYMPHOKINES
MATERIALS
MITOGENS
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PROTEINS
RADIOISOTOPES
SEPARATION PROCESSES
SULFUR ISOTOPES
VITAMINS
560300* - Chemicals Metabolism & Toxicology
550201 - Biochemistry- Tracer Techniques