Thin-layer chromatography of polyphosphoinositides from platelet extracts: interference by an unknown phospholipid
Journal Article
·
· Thromb. Res.; (United States)
Different ratios of radioactive polyphosphoinositides in platelets pulse-labelled with 32p-orthophosphate have been reported by various laboratories. We studied whether these differences originate from differences in methodology. Extracts of 32p-Pi labelled human platelets were prepared at various times after gel-filtration and phosphatidylinositol (PI)-, mono (PIP)- and bisphosphate (PIP2) were separated by thin-layer chromatography using four different solvent systems. The 32p-levels in PIP and PIP2 remained constant during one hour after gel-filtration, whereas 32p-PI increased continuously and more than doubled within the first h. In two of the systems PIP co-chromatographed with a radioactive compound which separated well from PIP in the two other systems. This unknown compound was also labelled with 3H-glycerol, 3H-inositol and 3H-arachidonic acid, but it was metabolically and functionally different from the polyphosphoinositides. Both the co-chromatography of this unknown phospholipid and the increase in 32p-PI in gel-filtered platelets can explain the difference in 32p-labelling in phosphoinositides reported in the literature.
- Research Organization:
- Univ. of Bergen, Norway
- OSTI ID:
- 6011324
- Journal Information:
- Thromb. Res.; (United States), Journal Name: Thromb. Res.; (United States) Vol. 3; ISSN THBRA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
38 RADIATION CHEMISTRY, RADIOCHEMISTRY, AND NUCLEAR CHEMISTRY
400702* -- Radiochemistry & Nuclear Chemistry-- Properties of Radioactive Materials
550201 -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD PLATELETS
BODY FLUIDS
CHEMICAL REACTION KINETICS
CHEMISTRY
CHROMATOGRAPHY
DAYS LIVING RADIOISOTOPES
ESTERS
ISOTOPES
KINETICS
LABELLING
LIGHT NUCLEI
LIPIDS
MATERIALS
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PHOSPHOLIPIDS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
RADIOCHEMISTRY
RADIOISOTOPES
REACTION KINETICS
SEPARATION PROCESSES
THIN-LAYER CHROMATOGRAPHY
400702* -- Radiochemistry & Nuclear Chemistry-- Properties of Radioactive Materials
550201 -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD PLATELETS
BODY FLUIDS
CHEMICAL REACTION KINETICS
CHEMISTRY
CHROMATOGRAPHY
DAYS LIVING RADIOISOTOPES
ESTERS
ISOTOPES
KINETICS
LABELLING
LIGHT NUCLEI
LIPIDS
MATERIALS
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PHOSPHOLIPIDS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
RADIOCHEMISTRY
RADIOISOTOPES
REACTION KINETICS
SEPARATION PROCESSES
THIN-LAYER CHROMATOGRAPHY