EGF- and PDGF-stimulated phosphorylation in young and senescent WI-38 cells

Journal Article · Fri Mar 01 04:00:00 EST 1991 · Experimental Cell Research; (USA)

DOI:https://doi.org/10.1016/0014-4827(91)90541-2· OSTI ID:6011143

Gerhard, G S; Phillips, P D; Cristofalo, V J ^[1]

---

1. Wistar Institute of Anatomy and Biology, Philadelphia, PA (USA)

We have examined the ability of epidermal growth factor (EGF) and platelet-derived growth factor (PDGF) to stimulate cultures of young and senescent WI-38 cells to carry out tyrosine-specific phosphorylation of their respective membrane receptors. Previously we reported no reduction in EGF-stimulated phosphorylation in plasma membrane preparations of senescent cells. In this study we found no reduction in PDGF-stimulated phosphorylation in plasma membrane preparations from senescent cells. Furthermore, we found no differences in the EGF- or PDGF-stimulated phosphorylation of their respective receptors in intact cells. These data support the previous findings that although the EGF receptor autokinase activity becomes highly labile during extraction and immunoprecipitation of senescent cells, in situ loss of receptor tyrosine kinase activity is apparently not responsible for the age-associated loss of mitogenic responsiveness.

• OSTI ID: 6011143
• Journal Information: Experimental Cell Research; (USA), Journal Name: Experimental Cell Research; (USA) Vol. 193:1; ISSN ECREA; ISSN 0014-4827
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMAL CELLS
BIOCHEMICAL REACTION KINETICS
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CELL MEMBRANES
CHEMICAL REACTIONS
GROWTH FACTORS
HYDROXY ACIDS
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
MEMBRANE PROTEINS
MEMBRANES
MITOGENS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PHOSPHORYLATION
PROTEINS
REACTION KINETICS
RECEPTORS
TRACER TECHNIQUES
TYROSINE