PDGF stimulation of Mueller cell proliferation: Contributions of c-JNK and the PI3K/Akt pathway

Journal Article · Fri Oct 09 04:00:00 EDT 2009 · Biochemical and Biophysical Research Communications

DOI:https://doi.org/10.1016/J.BBRC.2009.07.144· OSTI ID:22199833

Moon, Sang Woong ^[1]; Chung, Eun Jee ^[2]; Jung, Sun-Ah ^[3]

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1. Department of Ophthalmology, Seoul Paik Hospital, Inje University College of Medicine, Busan (Korea, Republic of)
2. Department of Ophthalmology, National Health Insurance Corporation, Ilsan Hospital, Gyounggi-do (Korea, Republic of)
3. Myunggok Eye Research Institute, Kim's Eye Hospital, Konyang University College of Medicine, Seoul (Korea, Republic of)

Platelet-derived growth factor (PDGF) has a critical role in proliferative vitreoretinopathy (PVR) as a chemoattractant and mitogen for retinal pigment epithelial cells and retinal glial cells. Here, we investigated the potential effects of PDGF on the proliferation of Mueller cells and the intracellular signaling pathway mediating these changes. PDGF induced Mueller cell proliferation and increased phosphorylation of the PDGF receptor (PDGFR), as shown by an MTT assay and immunoprecipitation analyses. Both effects were blocked by JNJ, a PDGFR-selective tyrosine kinase inhibitor. PDGF also stimulated phosphorylation of c-JNK and Akt. PDGF-induced Mueller cell proliferation was significantly reduced by pre-treatment with SP600125 and LY294002, inhibitors of c-JNK and Akt phosphorylation, respectively. Our findings collectively indicate that PDGF-stimulated Mueller cell proliferation occurs via activation of the c-JNK and PI3K/Akt signaling pathways. These data provide useful information in establishing the role of Mueller cells in the development of proliferative vitreoretinopathy.

• OSTI ID: 22199833
• Journal Information: Biochemical and Biophysical Research Communications, Journal Name: Biochemical and Biophysical Research Communications Journal Issue: 1 Vol. 388; ISSN 0006-291X; ISSN BBRCA9
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
CELL PROLIFERATION
GENE REGULATION
GROWTH FACTORS
PHOSPHORYLATION
RECEPTORS
RHODOPSIN
TYROSINE