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Application of intact algae to the biophotolysis

Conference · · Biotechnol. Bioeng. Symp.; (United States)
OSTI ID:6011005
The utilization of intact algae for the simultaneous light-activated production of molecular hydrogen and oxygen is discussed. Experimental data on the long-term stability and endurance of hydrogen and oxygen photoproduction by anaerobically adapted Chlamydomonas reinhardtii is presented. The general conclusion that can be derived from this work is that Chlamydomonas (and probably other microscopic eukaryotic algae) are extremely rugged organisms with respect to the biophotolysis problem. Working with a sample of wild-type Chlamydomonas reinhardtii, the simultaneous photoproduction of hydrogen and oxygen was observed for approximately 100 h. The bleached algae were then removed from the reactor and used as the inoculum in fresh growth medium. These second-generation cells regreened and were able to photoproduce hydrogen and oxygen initially at ten times the rate of the first generation cells and had better survivability characteristics as indicated by their chlorophyll content after 200 h. 2 figures.
Research Organization:
Oak Ridge National Lab., TN
OSTI ID:
6011005
Report Number(s):
CONF-820580-
Conference Information:
Journal Name: Biotechnol. Bioeng. Symp.; (United States) Journal Volume: 12
Country of Publication:
United States
Language:
English