Application of intact algae to the biophotolysis problem

Journal Article · Fri Jan 01 04:00:00 EST 1982 · Biotechnol. Bioeng. Symp.; (United States)

OSTI ID:5754160

Greenbaum, E

The utilization of intact algae for the simultaneous light-activated production of molecular hydrogen and oxygen is discussed. Experimental data on the long-term stability and endurance of hydrogen and oxygen photoproduction by anaerobically adapted Chlamydomonas reinhardtii is presented. The general conclusion that can be derived from this work is that Chlamydomonas (and probably other microscopic eukaryotic algae) are extremely rugged organisms with respect to the biophotolysis problem. Working with a sample of wild-type Chlamydomonas reinhardtii, the simultaneous photoproduction of hydrogen and oxygen was observed for approximately 100 h. The bleached algae were then removed from the reactor and used as the inoculum in fresh growth medium. These second-generation cells regreened and were able to photoproduce hydrogen and oxygen initially at ten times the rate of the first generation cells and had better survivability characteristics as indicated by their chlorophyll content after 200 h.

• Research Organization: Oak Ridge National Lab., TN
• DOE Contract Number: W-7405-ENG-26
• OSTI ID: 5754160
• Journal Information: Biotechnol. Bioeng. Symp.; (United States), Journal Name: Biotechnol. Bioeng. Symp.; (United States) Vol. 12; ISSN BIBSB
• Country of Publication: United States
• Language: English

Related Subjects

08 HYDROGEN
080106* -- Hydrogen-- Production-- Biosynthesis & Photochemical Processes
550700 -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
ALGAE
BASIC INTERACTIONS
BIOCONVERSION
BIOPHOTOLYSIS
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
CHLAMYDOMONAS
CHLOROPHYLL
DECOMPOSITION
ELECTROMAGNETIC INTERACTIONS
ELEMENTS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYDROGEN
HYDROGEN PRODUCTION
IN VIVO
INTERACTIONS
MICROORGANISMS
NONMETALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXYGEN
PARTICLE PRODUCTION
PHOTOCHEMICAL REACTIONS
PHOTOLYSIS
PHOTOPRODUCTION
PHOTOSYNTHESIS
PHYTOCHROMES
PIGMENTS
PLANTS
PORPHYRINS
SYNTHESIS
UNICELLULAR ALGAE