Increased intestinal permeability in endotoxic pigs. Mesenteric hypoperfusion as an etiologic factor

Journal Article · Fri Feb 01 04:00:00 EST 1991 · Archives of Surgery (Chicago); (USA)

DOI:https://doi.org/10.1001/archsurg.1991.01410260101014· OSTI ID:6007399

Fink, M P; Antonsson, J B; Wang, H L; Rothschild, H R ^[1]

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1. Univ. of Massachusetts Medical Center, Worcester (USA)

Infusing pigs with lipopolysaccharide (LPS) decreases superior mesenteric artery blood flow (Qsma), suggesting that mesenteric hypoperfusion may be responsible for LPS-induced alterations in gut mucosal permeability. To test this hypothesis, we studied four groups of anesthetized swine. Group 1 animals (N = 6) were infused with LPS (250 micrograms/kg over 1 hour beginning at 60 minutes) and continuously resuscitated with Ringer's lactate (48 mL/kg per hour). In group 2 (N = 5), Qsma was decreased by 50% by means of a mechanical occluder to mimic the LPS-induced alterations in Qsma observed in group I. Group 3 (N = 5) was included to document our ability to detect ischemia/reperfusion-induced alterations in mucosal permeability; in these pigs, Qsma was decreased in steps to zero flow (at 150 to 210 minutes) and then perfusion was restored (at 210 to 270 minutes). Pigs in group 4 (N = 6) served as normal controls; these animals were resuscitated with Ringer's lactate at the same rate as in group 1 but were not infused with LPS. To assess mucosal permeability, we measured plasma-to-lumen clearances for two markers, chromium 51-labeled edetic acid monohydrate (EDTA) and urea. Loading and maintenance infusions of the markers were given intravenously, and a 20-cm isolated segment of small intestine was continuously perfused at 2 mL/min with Ringer's lactate at 37 degrees C. Results were expressed as the ratio of the clearances for the two probes (CEDTA/CUREA). In group 3, CEDTA/CUREA was 999% +/- 355% of baseline at 270 minutes. In group 1, CEDTA/CUREA was 572% +/- 235% of baseline at 270 minutes. In groups 2 and 4, however, CEDTA/CUREA did not change significantly from the baseline value over the duration of the study. These data suggest that increased mucosal permeability after LPS is due to factors other than (or in addition to) mesenteric hypoperfusion.

• OSTI ID: 6007399
• Journal Information: Archives of Surgery (Chicago); (USA), Journal Name: Archives of Surgery (Chicago); (USA) Vol. 126:2; ISSN ARSUA; ISSN 0004-0010
• Country of Publication: United States
• Language: English

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550901* -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ANIMALS
BACTERIAL DISEASES
BETA DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BLOOD FLOW
CARBOHYDRATES
CARBOXYLIC ACIDS
CHELATING AGENTS
CHROMIUM 51
CHROMIUM ISOTOPES
DISEASES
DOMESTIC ANIMALS
EDTA
ELECTRON CAPTURE RADIOISOTOPES
ETIOLOGY
EVEN-ODD NUCLEI
INFECTIOUS DISEASES
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LIPIDS
LIPOPOLYSACCHARIDES
MAMMALS
MEMBRANES
MESENTERY
MUCOUS MEMBRANES
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
PERMEABILITY
POLYSACCHARIDES
RADIOISOTOPES
REACTION KINETICS
SACCHARIDES
SEROUS MEMBRANES
SWINE
TRACER TECHNIQUES
VERTEBRATES