Mucosal injury induced by ischemia and reperfusion in the piglet intestine: Influences of age and feeding

Journal Article · Sun Oct 01 04:00:00 EDT 1989 · Gastroenterology; (USA)

OSTI ID:5479392

Crissinger, K D; Granger, D N ^[1]

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1. Louisiana State Univ. Medical Center, Shreveport (USA)

The pathogenesis of neonatal necrotizing enterocolitis is unknown, but enteral alimentation, infectious agents, and mesenteric ischemia have been frequently invoked as primary initiators of the disease. To define the vulnerability of the intestinal mucosa to ischemia and reperfusion in the developing piglet, we evaluated changes in mucosal permeability using plasma-to-lumen clearance of chromium 51-labeled ethylenediaminetetraacetic acid in the ileum of anesthetized 1-day-, 3-day-, 2-wk-, and 1-mo-old piglets as a function of (a) duration of intestinal ischemia (20, 40, or 60 min of total superior mesenteric artery occlusion), (b) feeding status (fasted or nursed), and (c) composition of luminal perfusate (balanced salt solution vs. predigested cow milk-based formula). Baseline chromium 51-labeled ethylenediaminetetraacetic acid clearance was not significantly altered by ischemia, irrespective of duration, or feeding in all age groups. However, clearances were significantly elevated during reperfusion after 1 h of total intestinal ischemia in all age groups, whether fasted or fed. Reperfusion-induced increases in clearance did not differ among age groups when the bowel lumen was perfused with a balanced salt solution. However, luminal perfusion with formula resulted in higher clearances in 1-day-old piglets compared with all older animals. Thus, the neonatal intestine appears to be more vulnerable to mucosal injury induced by ischemia and reperfusion in the presence of formula than the intestine of older animals.

• OSTI ID: 5479392
• Journal Information: Gastroenterology; (USA), Journal Name: Gastroenterology; (USA) Vol. 97:4; ISSN GASTA; ISSN 0016-5085
• Country of Publication: United States
• Language: English

Related Subjects

550901* -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AGE DEPENDENCE
AMINO ACIDS
ANIMALS
BETA DECAY RADIOISOTOPES
BODY
CARBOXYLIC ACIDS
CARDIOVASCULAR DISEASES
CHELATING AGENTS
CHROMIUM 51
CHROMIUM ISOTOPES
DIGESTIVE SYSTEM
DIGESTIVE SYSTEM DISEASES
DISEASES
DOMESTIC ANIMALS
EDTA
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
GASTROINTESTINAL TRACT
INTERMEDIATE MASS NUCLEI
INTESTINES
ISCHEMIA
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MEMBRANES
MUCOUS MEMBRANES
NEONATES
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PATHOGENESIS
PERFUSED ORGANS
RADIOISOTOPES
SWINE
TRACER TECHNIQUES
VASCULAR DISEASES
VERTEBRATES