Recovery of the proliferative and functional integrity of mouse bone marrow in long-term cultures established after whole-body irradiation at different doses and dose rates

Journal Article · Fri Feb 01 04:00:00 EST 1991 · Experimental Hematology (Lawrence, Kansas); (USA)

OSTI ID:6006073

Bierkens, J G; Hendry, J H; Testa, N G ^[1]

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1. Paterson Institute for Cancer Research, Manchester (England)

Injury inflicted upon the bone marrow stroma following whole-body irradiation and its repair over a 1-year period has been assessed in murine long-term bone marrow cultures established at increasing time intervals after irradiation. Different doses at different dose rates (10 Gy at 0.05 cGy/min, 4.5 Gy and 10 Gy at 1.6 cGy/min, and 4 x 4.5 Gy (3 weeks between doses) at 60 cGy/min) were chosen so as to maximize differences in effect in the stroma. The cellularity of the adherent layer in long-term cultures established 1 month after irradiation was reduced by 40%-90% depending on the dose and dose rate. Simultaneous with the poor ability of the marrow to form adherent layers, the cumulative spleen colony-forming unit (CFU-S) and granulocyte-macrophage colony-forming cell (GM-CFC) production over a 7-week period was reduced to 0% and 30% of control cultures, respectively. The slow recovery of the adherent layer was paralleled by an increase in the numbers of CFU-S and GM-CFC in the supernatant. Cultures established from repeatedly irradiated mice performed poorly over the entire 1-year period. Whereas the regeneration of the stroma was near complete 1 year after irradiation, the CFU-S and GM-CFC levels reached only between 50% and 80% of control cultures, respectively. Also, the concentration of CFU-S and GM-CFC in the supernatant remained persistently lower in cultures established from irradiated mice as compared to control cultures. The levels of sulfated glycosaminoglycans, which have been implicated in the establishment of the functional integrity of the microenvironment, were not reduced in the adherent layers at any time after irradiation. These results indicate that the regeneration of the stroma is accompanied by an incomplete recovery of active hemopoiesis in vitro.

• OSTI ID: 6006073
• Journal Information: Experimental Hematology (Lawrence, Kansas); (USA), Journal Name: Experimental Hematology (Lawrence, Kansas); (USA) Vol. 19:2; ISSN EXHMA; ISSN 0301-472X
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BONE MARROW CELLS
CELL PROLIFERATION
CONNECTIVE TISSUE CELLS
DOSE-RESPONSE RELATIONSHIPS
DOSES
EXTERNAL IRRADIATION
GLYCOPROTEINS
IRRADIATION
MAMMALS
MICE
ORGANIC COMPOUNDS
PROTEINS
RADIATION DOSES
RECOVERY
REPAIR
RODENTS
SOMATIC CELLS
VERTEBRATES
WHOLE-BODY IRRADIATION