Bioconversion of. cap alpha. -(/sup 14/C)Zearalenol and. beta. -(/sup 14/C)Zearalenol into (/sup 14/C)Zearalenone by Fusarium roseum Gibbosum

Journal Article · Fri Jun 01 04:00:00 EDT 1984 · Appl. Environ. Microbiol.; (United States)

OSTI ID:6005501

Richardson, K E; Hagler, Jr, W M; Hamilton, P B

Cultures of Fusarium roseum Gibbosum on rice were treated with (/sup 14/C)zearalenone, ..cap alpha..-(/sup 14/C)zearalenol, or ..beta..-(/sup 14/C)zearalenol to determine whether a precursor-product relationship exists among these closely related fungal metabolites. Culture extracts were purified by silica gel column chromatography and fractionated by high-pressure liquid chromatography, and the level of radioactivity was determined. Within 7 days, the ..beta..-(/sup 14/C)zearalenol was converted to zearalenone, and no residual ..beta..-(/sup 14/C)zearalenol was detectable. Most of the ..cap alpha..-(/sup 14/C)zearalenol added was also converted into zearalenone within 14 days. In cultures treated with (/sup 14/C)zearalenone, no radioactivity was noted in any other components.

• Research Organization: North Carolina State Univ., Raleigh
• OSTI ID: 6005501
• Journal Information: Appl. Environ. Microbiol.; (United States), Journal Name: Appl. Environ. Microbiol.; (United States) Vol. 47:6; ISSN AEMID
• Country of Publication: United States
• Language: English

Related Subjects

550701* -- Microbiology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACETATES
BIOCONVERSION
CARBON 14 COMPOUNDS
CARBOXYLIC ACID SALTS
CELL CULTURES
CHROMATOGRAPHY
FUNGI
FUSARIUM
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIQUID COLUMN CHROMATOGRAPHY
METABOLISM
METABOLITES
PARASITES
PLANTS
SEPARATION PROCESSES
TRACER TECHNIQUES