Triacylglycerol secretion in rats: validation of a tracer method employing radioactive glycerol
A two-compartment model was developed to analyze the temporal changes in plasma triacylglycerol (TG)-specific radioactivity after injection of (2-/sup 3/H)glycerol into rats. The analysis, which yielded fractional rate constants of TG secretion, was tested in rats fed diets either adequate or deficient in essential fatty acids (EFA) and containing either glucose, fructose or sucrose as the dietary carbohydrate. The method of analysis appeared valid, first, because of a close agreement between experimental and computer-fitted TG-specific radioactivity curves, and second, because the fractional rate constants obtained were quite similar to fractional rate constants determined previously by the Triton WR-1339 technique in rats maintained on identical diets. The results show that EFA deficiency increased the fractional rate constant of TG secretion 1.7-, 1.8- and 3.3-fold and the rate of TG secretion 1.8-, 1.6- and 1.4-fold when the dietary carbohydrate was glucose, sucrose and fructose, respectively, in comparison with control rats fed diets supplying these same carbohydrates but adequate in EFA. In the latter groups, the rates of plasma TG secretion were in the range of 0.14-0.17 mg/min per 100 g body weight, and the rate of secretion in the fructose-fed rats was only 20% higher than in the glucose-fed rats.
- Research Organization:
- Univ. of California, Berkeley
- OSTI ID:
- 6001475
- Journal Information:
- J. Nutr.; (United States), Vol. 114:10
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
GLYCEROL
SECRETION
NUTRITIONAL DEFICIENCY
BIOLOGICAL EFFECTS
BIOLOGICAL MODELS
BLOOD PLASMA
CARBOHYDRATES
CARBOXYLIC ACIDS
CHEMICAL ANALYSIS
RADIOACTIVITY
RATS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
VALIDATION
ALCOHOLS
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BODY FLUIDS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
MATERIALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
RODENTS
TESTING
VERTEBRATES
550201* - Biochemistry- Tracer Techniques