Coupling of glucose oxidase and potassium ferrocyanide as a basis for a simple colorimetric estimation of glucose

Conference · Wed Jan 01 04:00:00 EST 1986

OSTI ID:6000274

Woodward, J; Baker, W J

The coupling of glucose oxidase and Fenton's reagent for a simple, inexpensive assay of glucose has been described. In the reaction, hydrogen peroxide, formed as a result of the action of glucose oxidase on glucose, oxidizes ferrous to ferric sulfate which results in an increase in the absorbance of ultraviolet light at 340 nm. It was proposed that the assay would be useful to measure the activity of enzymes such as cellulase and ..beta..-glucosidase. Potassium ferrocyanide (colorless) when oxidized to potassium ferricyanide results in a yellow color with an absorbance maximum at 420 nm. Described here are some preliminary results indicating that the interaction between hydrogen peroxide and potassium ferrocyanide results in an increase in absorbance which is maximal at 420 nm. A simple assay for glucose based upon this interaction might be useful under some circumstances. 3 refs., 4 figs.

• Research Organization: Oak Ridge National Lab., TN (USA)
• DOE Contract Number: AC05-84OR21400
• OSTI ID: 6000274
• Report Number(s): CONF-860508-1; ON: DE86009148
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
090222 -- Alcohol Fuels-- Preparation from Wastes or Biomass-- (1976-1989)
550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ABSORPTION SPECTROSCOPY
ALDEHYDES
BIOASSAY
CARBOHYDRATES
CELLULASE
COMPLEXES
ENZYME ACTIVITY
ENZYMES
FERROCYANIDES
GLUCOSE
GLUCOSIDASE
GLYCOSYL HYDROLASES
HEXOSES
HYDROLASES
IRON COMPLEXES
MONOSACCHARIDES
O-GLYCOSYL HYDROLASES
ORGANIC COMPOUNDS
SACCHARIDES
SPECTROSCOPY
TRANSITION ELEMENT COMPLEXES