Characterization and comparison of a Neurospora crassa RNase purified from cultures undergoing each of three different states of derepression

Journal Article · Wed Feb 01 04:00:00 EST 1984 · J. Bacteriol.; (United States)

OSTI ID:6000108

Lindberg, R A; Drucker, H

Extracellular RNase N/sub 4/ from Neurospora crassa is derepressible by limitation of any of the three nutrient elements obtainable from RNA. The authors have purified and characterized the enzyme from cultures grown under each of the three states of derepression. The purification procedure consisted of an ultrafiltration step, cation-exchange chromatography, and gel filtration. The authors found only one enzyme (N/sub 4/) that hydrolyzed RNA at pH 7.5 in the presence of EDTA in culture filtrates from nitrogen-, phosphorus-, or carbon-limited cells. In all three cases, the enzymes were identical by polyacrylamide gel electrophoresis (M/sub r/ approx.9500) and by gel filtration (M/sub r/ approx.10,000). There were no differences in thermal stability or pH optimum; all three cross-reacted with antibody to the nitrogen-derepressed enzyme in interfacial ring and in Ouchterlony tests. Digestion of homopolyribonucleotides indicated that N/sub 4/ preferentially cleaved phosphodiester bonds adjacent to guanine residues. Results indicate that the enzymes are very similar or identical and are probably products of the same gene. N/sub 4/ appears to be homologous to guanine-specific RNases from other fungal sources. 25 references, 4 figures, 1 table.

• Research Organization: Pacific Northwest Lab., Richland, WA
• DOE Contract Number: AC06-76RL01830
• OSTI ID: 6000108
• Journal Information: J. Bacteriol.; (United States), Journal Name: J. Bacteriol.; (United States) Vol. 157:2; ISSN JOBAA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
BIOSYNTHESIS
CARBON
CARBOXYLIC ACIDS
CHELATING AGENTS
CHROMATOGRAPHY
COMPARATIVE EVALUATIONS
DATA
EDTA
ELECTROPHORESIS
ELEMENTS
ENZYMES
ESTERASES
EXPERIMENTAL DATA
FILTRATION
FUNGI
HYDROLASES
INFORMATION
ION EXCHANGE CHROMATOGRAPHY
NEUROSPORA
NITROGEN
NONMETALS
NUCLEIC ACIDS
NUMERICAL DATA
ORGANIC ACIDS
ORGANIC COMPOUNDS
PH VALUE
PHOSPHODIESTERASES
PHOSPHORUS
PLANTS
PURIFICATION
RNA
RNA-ASE
SEPARATION PROCESSES
SYNTHESIS