Phytochrome from green plants: Assay, purification, and characterization

Technical Report · Mon Jun 10 04:00:00 EDT 1991

DOI:https://doi.org/10.2172/5999907· OSTI ID:5999907

Quail, P H ^[1]

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1. California Univ., Berkeley, CA (United States). Dept. of Plant and Soil Biology Agricultural Research Service, Albany, CA (United States). Plant Gene Expression Center

This funding period was directed at developing an in-depth molecular analysis of the low-abundance, 118,000 M{sub r} green-tissue phytochrome that had at that time been relatively recently identified as being distinct from the better characterized 124,000 M{sub r} phytochrome abundant in etiolated tissue. The specific objectives as stated in the original proposal were: (1) To generate monoclonal antibodies specific to the 118,000 M{sub r} green-tissue phytochrome. (2) To develop additional and improved procedures to permit progress toward the ultimate goal of purifying green-tissue phytochrome to homogeneity. (3) To initiate an alternative approach to determining the structural properties of green-tissue phytochrome by isolating and sequencing cDNA cones representing the 118,000 M{sub r} green-tissue polypeptide in Avena. This approach is based on and will test hypothesis that the 118,000 M{sub r} polypeptide is encoded by a gene(s) distinct from those encoding etiolated-tissue 124,000 M{sub r} phytochrome. (4) To utilize any such 118,000 M{sub r} phytochrome specific cDNA clones as hybridization probes to begin to investigate the structure, organization, and regulation of the corresponding gene(s) in Avena. (5) To begin to investigate the possible presence in other higher plant and algal species of sequences homologous to the 118,000 M{sub r} Avena polypeptide using the Avena clones at hybridization probes. Most of these objectives have been accomplished, at least in principle, although the major breakthrough establishing that phytochrome is encoded by a multigene family came from the use of Arabidopsis rather than Avena. Similarly, much of the characterization subsequent to this discovery has been performed in Arabidopsis and rise as model dicot and monocot systems, respectively, rather than Avena. 13 refs., 9 figs.

• Research Organization: California Univ., Berkeley, CA (United States). Dept. of Plant Biology; Agricultural Research Service, Albany, CA (United States). Plant Gene Expression Center
• Sponsoring Organization: DOE; USDOE, Washington, DC (United States)
• DOE Contract Number: FG03-87ER13742
• OSTI ID: 5999907
• Report Number(s): DOE/ER/13742-5; ON: DE92003396
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
550400 -- Genetics
59 BASIC BIOLOGICAL SCIENCES
ANTIBODIES
ARABIDOPSIS
BIOASSAY
BIOCHEMISTRY
BIOLOGY
BOTANY
CEREALS
CHEMISTRY
CLONING
DNA HYBRIDIZATION
DNA SEQUENCING
DNA-CLONING
DOCUMENT TYPES
GENE REGULATION
GRAMINEAE
HYBRIDIZATION
LILIOPSIDA
MAGNOLIOPHYTA
MAGNOLIOPSIDA
MOLECULAR BIOLOGY
MONOCLONAL ANTIBODIES
OATS
ORGANIC COMPOUNDS
PHYTOCHROMES
PIGMENTS
PLANTS
PROGRESS REPORT
PROTEINS
PURIFICATION
RICE
STRUCTURAL CHEMICAL ANALYSIS