Phytochrome from green plants: assay, purification and characterization. Progress report, June 1, 1984-May 1, 1985

Technical Report · Tue Jan 01 04:00:00 EST 1985

OSTI ID:6168837

Quail, P H

The major differences that we have found between the phytochrome extracted from green and from etiolated tissue has been documented in a paper that is now in press. In addition to the previously reported spectral and immunochemical differences, we have now established: (a) that the predominant (approx. 80% of total) phytochrome polypeptide in green tissue has a relative molecular mass (Mr) of 118,000; (b) that the proteolytic peptide map of this 118,000-Mr species differs considerably from that of 124,000-Mr phytochrome from etiolated tissue; (c) that the green-tissue, 118,000-Mr polypeptide carries only one of three spatially separate epitopes that are present on etiolated-tissue phytochrome (i.e., an epitope at the carboxy-terminal end recognized by Type 3 monoclonal antibodies); and (d) that the minor phytochrome species in green tissue (approx. 20% of total) resembles that in etiolated tissue in that it is 124,000-Mr and is immunoprecipitable with polyclonal, anti-etiolated-oat antibodies, thereby accounting for the previously observed limited population of immunoprecipitable activity in green extracts. Present efforts are being concentrated on purification of the new phytochrome species. 3 references.

• Research Organization: Wisconsin Univ., Madison (USA)
• DOE Contract Number: AC02-81ER10903
• OSTI ID: 6168837
• Report Number(s): DOE/ER/10903-5; ON: DE85006115
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ANTIBODIES
COMPARATIVE EVALUATIONS
MOLECULAR WEIGHT
MONOCLONAL ANTIBODIES
ORGANIC COMPOUNDS
PEPTIDES
PHYTOCHROMES
PIGMENTS
POLYPEPTIDES
PROTEINS
PURIFICATION